<8) MycoKeys MycoKeys 121: 111-142 (2025) DOI: 10.3897/mycokeys.121.157714 Research Article Four new species of the lichen genus Diorygma (Graphidaceae, Ostropales) from Guizhou, China Wei Wu'®, Shu-Hao Jiang”®, Lin-Shan Chai'®, He-Yun Bo'™®, Ruvishika S. Jayawardena*“*®, Shao-Bin Fu™®, Qing-Feng Meng®® a fF Ww NY -| School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou Province 563000, China College of Agriculture and Biology, Liaocheng University, Liaocheng, Shandong Province 252059, China Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand School of Public Health, Zunyi Medical University, Zunyi, Guizhou Province 563000, China Corresponding authors: Shao-Bin Fu (fushb@126.com); Qing-Feng Meng (qfmeng@126.com) OPEN Qaccess This article is part of: Exploring the Hidden Fungal Diversity: Biodiversity, Taxonomy, and Phylogeny of Saprobic Fungi Edited by Samantha C. Karunarathna, Danushka Sandaruwan Tennakoon, Ajay Kumar Gautam Academic editor: Danushka Sandaruwan Tennakoon Received: 2 May 2025 Accepted: 21 July 2025 Published: 22 August 2025 Citation: Wu W, Jiang S-H, Chai L-S, Bo H-Y, Jayawardena RS, Fu S-B, Meng Q-F (2025) Four new species of the lichen genus Diorygma (Graphidaceae, Ostropales) from Guizhou, China. Mycokeys 121: 111-142. https://doi. org/10.3897/mycokeys.121.157714 Copyright: © Wei Wu et al. This is an open access article distributed under terms of the Creative Commons Attribution License (Attribution 4.0 International - CC BY 4.0). Abstract Four new species of the lichen genus Diorygma from China are described based on mor- phological, chemical, and phylogenetic evidence. Phylogenetic analyses were conduct- ed using both RAXML and Bayesian posterior probability inference, based on combined LSU and mtSSU sequences. The characteristic compounds were analyzed by colorimet- ric reactions and thin-layer chromatography. Diorygma guizhouense is characterized by small apothecia, a narrow to slightly open disc covered with white pruina, and the presence of stictic, salazinic, and norstictic acids. Diorygma leigongshanense is char- acterized by small, oval apothecia, a disc surrounded by entire, raised, and widely open thalline margins covered with thin, pale yellowish pruina, and the same chemical sub- stances as D. guizhouense. Diorygma locitonitrus is distinguished by the presence of sal- azinic acid and hyaline, richly muriform ascospores, notably with distinctly smaller pe- ripheral cells compared to the central cells. Diorygma weii is characterized by stellately branched apothecia with a closed to slit-like disc, a milky white thallus tinged with green, and the presence of only norstictic acid. Detailed morphological descriptions and illus- trations of the new species are provided, along with a comprehensive species checklist highlighting the diagnostic characteristics of the known species in this genus. Key words: 4 new species, lichenized fungi, morphology, muriform, phylogeny Introduction Diorygma was described by Eschweiler (1824), who mentioned Opegrapha hi- eroglyphica as a member of this genus but did not formally designate it as the type species. Later, Staiger (2002) synonymized Opegrapha hieroglyphica as Diorygma hieroglyphicum and designated it as the lectotype for Diorygma. The genus is widely distributed, primarily in tropical regions, and often thrives on the sheltered or overhanging sides of trees (Staiger 2002; Kalb et al. 2004). It is characterized by a crustose, white to pale olive-green thallus and lirelliform to irregularly rounded ascomata with a pruinose disc. The exciple is uncarbon- 111 Wei Wu et al.: Diorygma from China ized or slightly carbonized, and the hymenium is hyaline, not inspersed, with branched or anastomosing paraphyses that have a thick gelatinous wall. The asci are clavate and contain 1-8 spores. The ascospores are hyaline (rarely brownish), transversely septate to mostly muriform (Li and Jia 2016). Chem- ically, the genus produces compounds such as norstictic acid, stictic acid, or the protocetraric acid complex (Kalb et al. 2004; Aptroot et al. 2023). Kalb et al. (2004) provided a monograph on the genus Diorygma, which in- cluded 24 recognized species, a detailed taxonomic key, and a phylogenetic tree based on LSU sequences. Subsequently, Sharma and Makhija (2009a, 2009b) described four additional species characterized by the presence of norstictic and salazinic acids as the major secondary metabolites. Later, Sharma and Khadilkar (2012) reported four more new species from India, two of which exclusively contain norstictic and salazinic acids. Feuerstein et al. (2014) ex- panded the diversity of the genus by describing three new species and provid- ing a revised global key encompassing 52 species. Numerous additional taxa have been discovered and reported, further increasing diversity (Caceres 2007; Makhija et al. 2009; Sharma and Makhija 2009a; Lima et al. 2013; Feuerstein et al. 2014; Sutjaritturakan et al. 2014; Aptroot et al. 2023, 2024). According to the latest phylogenetic research by Ansil et al. (2023), D. dandeliense B.O. Sharma & Khadilkar is a synonym of D. karnatakense B.O. Sharma & Khadilkar. Currently, 89 species of the genus Diorygma are recognized worldwide (Table 2). Molecu- lar data for 15 known species and 4 novel species are available (Table 1). During a field survey of lichens in Guizhou Province, we discovered several specimens that formed phylogenetically distinct clades within Diorygma. Fol- lowing detailed morphological and chemical analyses and comparison with all known species, we propose these as four new species. Material and methods Sample collection and morphological observations Specimens were collected from the Leigong Shan National Nature Reserve in Leis- han County and the Yueliangshan Nature Reserve in Congjiang County, Guizhou Province, China. All voucher specimens are deposited in the Lichen Herbarium of the Kunming Institute of Botany (KUN-L), Chinese Academy of Sciences, Yunnan, China. External morphological characteristics of the thallus and ascomata were examined using a stereomicroscope (OLYMPUS SZX16, Japan) and photographed with a fitted digital camera (AOR B32, China). Anatomical features, including the exciple, hymenium, paraphyses, asci, and ascospores, were observed using a light microscope (OLYMPUS BX53, Japan) based on hand-cut longitudinal sections of apothecia manually prepared with a razor blade. The sections were immersed in distilled water, and images were captured with a digital camera (OLYMPUS DP72, Japan). Lugol's iodine solution (I) was used to stain and examine the hymenium. Photographic plates were assembled using Adobe Photoshop CC 2019 (Adobe Systems, USA). Measurements were conducted using ImageJ software (v. 1.50d) and are presented as (min-—) (x — SD) — (x + SD) (—max), where x is the arithmetic mean and SD is the standard deviation (rounded to the nearest 0.5 um), followed by the number of observations (n) when n = 10. The terms ‘min’ and ‘max’ repre- sent the extreme values observed (Zhurbenko and Diederich 2024). MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 112 Wei Wu et al.: Diorygma from China Table 1. Taxon name, voucher/culture, and GenBank accession numbers used in this study. Newly generated sequences are shown in bold, and “"” indicates holotype strains. Absence of GenBank accession numbers is indicated by “NA”. Taxon Diorygma antillarum D. antillarum D. antillarum D. antillarum D. circumfusu D. defectoisidiatum D. defectoisidiatum D. guizhouense D. hieroglyphicum D. junghuhnii D. junghuhnii D. junghuhnii D. junghuhni D. junghuhnii D. junghuhnii D. karnatakense D. karnatakense D. karnatakense D. karnatakense D. karnatakense D. leigongshanense D. locitonitrus D. microsporum D. minisporum D. minisporum D. aff. minisporum D. tibellii D. poitaei D. pruinosum D. pruinosum D. sipmanii D. tiantaiense D. toensbergianum Diorygma sp. Diorygma sp. Diorygma sp. D. weii Glyphis cicatricosa G. cicatricosa Locality Brazil USA El Salvador El Salvador Australia Brazil Brazil China French Australia Fiji Australia Brazil India India India India India India India China China USA Kenya USA South Africa Nicaragua Nicaragua Australia Australia Costa Rica China Brazil Australia Fiji Fiji China El Salvador Kenya Voucher/Strain Nelsen 4185 (F) Nelsen 4037 (F) Liicking 33018 (F) Liicking 33019 (F) Kalb 33922 (Herb. Kalb) Caceres & Aptroot 28966a’ (ISE) Caceres & Aptroot 28966b (ISE) L 0093724" (KUN) Wirth 26647 Kalb 33937 (Herb. Kalb) Lumbsch 20539 (F) Kalb 33931 (Herb. Kalb) Kalb 33254 (Herb. Kalb) Mycobiont (MSSRF) Thallus (MSSRF) 21.26 (AMH) 21.52 (AMH) 21.54 (AMH) 21.55 (AMH) 21.60 (AMH) L 0093725" (KUN) L 0093723" (KUN) Liicking 26504 (F) Lumbsch 19543v (F) Liicking 26564 (F) Medeiros 2106 (DUKE) Liicking 28533 (F) Liicking 28538 (F) Mangold 28g (F) Kalb 26578 (Herb. Kalb) Licking 14011 (F) Jia ZJ19123' (LCUF) Caceres & Aptroot 42003' (ISE) Lumbsch 19082I (F) Lumbsch 205011 (F) Lumbsch 20513a (F) L 0093727" (KUN) Liicking 28047 (F) Lumbsch 195280 (F) Chemical component analysis GenBank accessions number mtSSU JX046451 JX046452 JX046453 JX046454 DQ431963 OR270821 OR270822 PQ691396 NA DQ431962 JX421023 NA NA MN944821 MN944822 OP235521 OP235522 OP235523 OP235524 OP235525 PQ847480 PQ847478 JX421024 HQ639598 NA ON507279 JX421025 HQ639596 NA NA DQ431961 NA OR270820 NA NA NA PQ847479 HQ639610 JX421062 LSU JX046464 JX046465 NA JX046467 NA NA NA PQ691395 AY640015 NA JX421474 AY640017 AY640016 NA NA OP235516 OP235517 OP235518 OP235519 OP235520 NA PQ847477 NA HQ639626 HQ639665 ON507251 JX421475 HQ639627 JX421476 AY640014 AY640020 MW750692 NA JX421479 JX421478 JX421477 NA JX421505 JX421503 The color reactions of the thallus and medulla were tested using the following reagents: 10% potassium hydroxide (KOH, K), saturated sodium hypochlorite solution (NaCIO, C), and a saturated solution of p-phenylenediamine in 95% MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 113 Wei Wu et al.: Diorygma from China ploe oWeNed010Id :O7 1 ‘-9y ‘-9 (€ LOZ) ‘aBuelo-moj|ah +dd ‘Moj|aA jeyjUao = ‘Je Ja lusor Anybys +4 40 —y snyyeuy JeJayduad ‘wuojunuu —|/> pue —| ‘_AQ) ‘aBueso +¢ ‘pal Udy} MoO]JaA + ‘—O snyyjeyjoud pue JOUL “D8 SNIJEUL OL YUM pajoajep Huron ‘jooudy ulloaz pue UOUeL}e YIM (uleA) Wwindeljjue (ZL0Z) Ajjeuonppe Ajaues ‘pioe uojjeyjodiay = Je 1a MeyneW 912}990} 01d ]NOYUM “ule, LUNe]/Ue ‘(600Z) ‘Ie JO YUM ‘[Jofeu] spioe uoJDapoly) = }@ jOoNdYy O1}91}SOU pue dIuIzejes (e0e1}) ploe oNONSOdAY pue ploe ononsiou ‘(dofewiqns) ploe olulzejesuod ‘(JOuIW) jejjuad = jeJaydued (yLOZ) 139 =| ploeonoNsoydAso ‘ofew) | 4ejND0] OL-8/8Z-0Z ueyeinyefing | sploe oiulzeyes pue onons ‘WWOyN Ajyou Bay INN (vZ0Z) 11200] 7—G Bulureyuoo eqje eulydesy = 2 Ja jooNndy ‘9SJOASUBL} (6002) (iofew) spioe o101)s JeU90 = ‘Je ja efluyeW | pue ‘ononsoydAlo ‘ononsuoo | jesayduad ‘wwojsuNuw (4ofew) spioe (6002) olulzejes pue ‘ononsiou jesjUao = ‘je 18 eflyyew ‘onons|Ayjauu ‘ononsoydAuo =| jeseyduad ‘won ploe onons ‘(moja YBUg +AN (Z00Z) sau80e9 | ‘AJUO Snyjey) PUOyUeXAaY)| UWWJOJUNUWW (ZL0Z) (pad +d ‘Mo}jaA Jey|IPeuy +>) Juasaid sploe o1011s pue JeujUad = pue euwweYys ‘ouesa0}OId ‘oNONSO}dAID =| jesayduad ‘WwwoJNuw ploe AjsnouaboJoe ‘wun oes}900}01dodAyjAUjauu Lx G'p ‘elpluoo -0-7 |euolippe WoL IOeq (v00z) UUM UOHda]}09 adAy jetjUad > |esayduad ‘PaSJOLULU! ‘je Ja qey ‘ploe OUWeu800} 01d ‘WWOjUNUU Ajasuap (Z LOZ) ybuis (4oulLu) ploe 9101;sU0D azis jenba jo pue ybuls ‘Gofeuu) pioe ojos $9|NooJ ||P ‘WOJ NW S3]0N SoOUdJOJoY Adysiwiay9 eydas lun 78-9 x GGZ-O9L wun /6-0€ x OLL-06 wun €€ x 06 lun 9¢-ZL x 66-99 wun /Z-81L x OSL-SEL wun yL-OL x 09-07 wun /€-02 x GZL-Z8 wun G9-0r x 00Z-OVL Ov-92 x OC L—-06 (-9/) win/azis you, url O€-0Z ‘umodg ajed 0} auljeAy anjq ‘paziuoqueo-uoUu Apyeam ‘aurjua ‘padojaaap ANIG-J9JOIA +] ‘L | JO ang +} Ajsood ajdioxa uaas you elpiudAd pue jose JOJOIA +] ‘L aniq +1 L J9[OIA +] 8-7 ani +1 JOJOIA +] ‘T—L an|q +! jUaBJaAUOD a|dIoxa anjq -JB[OIA +] ‘8-9 SS9]JO]OO JO[OIA an|q +| ‘SMOl ul paBuewe g an|q +! jUaBJaAUOD a|dIoxa ysiniq J9[O1A-YSIN|q Apyeam pasiuoqied-ysiumolq Apyeam +] JO -] ‘1 +| 10 -| ‘\UaHJaAIp 3|dloxa jUaBJaAIp Ajybus 0} JUabsaAUod JBJOIA +] ‘Q-7 JBJOIA +|__| ‘Pazluoqueoun ajdioxa ‘uinu sajods | wuiniualwwiAH | ajdioxg ‘saioads ewBbAJOIg Jo UOSIWedWUOD jeoIHojoydo\ *Z aIGeL paesodxa Ja}e| ‘pasojo WLU £60 Ajyeniul osip -SS'0xG'€-07% payouerig Ajasueds Jo peayoueiqun {WeOlpul|Ao ‘SnoJeuunu elpisipnasd pauado Ajapim Ajase ‘pjo uayM uado F Hulluooaq ‘BunoA UUW UdYM dy WIS OSIP | CO-LOxGL-c0 UIU} ‘8}9|dLUOOU! Ajeseq uunisaujed o> ![-UNS >I] SANSSIJ “LULU ‘MOJJEU SIP G0-20x€-S0 OIA ayels-E-Z ‘MO.UBU OSIP ‘WU L-Z'0 x 9-G'0 auejd “WW /'0-7'0 x 0} 8BABDUOD OSIP | G*L—-G'Q ‘ayeHaibbe uado F Ajaies ‘a>! -}|S ‘MO.ueU OSIP WIL G*L-L ys daap e Aq payeiedas ualjo uado osip ‘WWW L-G'Q x G—L oy! AUIS 0} pasojo osIp osid eyeWwoosy 114 INH 3 lysor "S uayore eWUBAIOIG | OL eye|q SPAY 8 Bulryjony ‘Uas|aN (‘ule/) wuneyjijue eUBAIOIG | 6 q\ey 3 ueyeinuehns ‘7 windueonsnbue ewbAI0Ig| 8 yooudy 3 sala0e9 ‘W ‘bupjon] (Buy "INW) wingje ewbfuoig |Z BUWEYS ‘O'd 8 SJEUYOD ‘eflyye|\| SUadSauAoge ewbAI0lIg| 9 PUUIEUS 0'8 B BJeUyO ‘ellyyen suaosesauloog)e ewbAI0Ig) Ss Huron] y sasaoe9 asuaobeje ewhKI0ig |) Jey|IPEYy 8 BLUES ‘O'd asuaquunbe ewhXioig) §€ XI 8 ables ‘qley wunueoje ewbhAl0ig| Z yBuls dy 3 yBuls Idysnd (‘11NS) wnjose ewbAloig|) | aweu saiseds MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China (vLOZ) 1219 ueyeinqueling (vo0z) ‘Je 19 qey (vLOZ) 1218 ueyeunqueling (0z0z) aley (€€8L) Jajlamyosy (vLOZ) Aeneas pue Aaneas (6002) eIpisi Uolssassod | XI|q pue JayoV S210N saqualajoy saoue}sqns uMouUN OM, pue (4ouluu) ploe o101}s]AUouU ‘Gofeww) ploe onONsodAy ‘(dofeuw) ploe ojulzejes (e0e1) Ajjensn) ploe anos ‘(e0e4}) ploe Od1ssOUUOD ‘GouILW) ploe o1]011Ss0}dAN0 ‘dofew) ploe oNONSJOU (4ouluW) ploe o10I]S JOU pue (Jofeuw) ploe ojuizejes (4ofetugns) pice 91)91]s ud ‘GoulW) ploe oleizzeBauauw ‘(lofewu) ploe o0Ns ploe on0Ns :O71 ‘ebueso +d ‘-9 ‘-Oy ‘molak +4 (Jour) pide o191}s1oUU0D ‘Gofeww) ploe o01NSsJOU Alysiwayo jes}uao = |e1aydisad 4e|NdO| OL-v/LL-VL ‘WWOJNUU A|YOU BUILUN] WOJUd} YUM Je|NDO| EZ-EL ‘azeydas Ajassansued} Jejnoo| OL-Z x 02-6L ‘We1jUBO > |e1aydiad ‘UWOJNUU A|YOU (¢ Wopyjas Alan) Z JO | ul wunjdas jeulpny6uo] L-0 pue aSJaASUBI] G—¢ YUM WOsUuNWugns Pe}|29-OL—-8 WwwoJNuu AjYyou eydas wn 0€-02 x 0£-09 un 9L-G’8 x 00L-09 wun Qv—-ZE x OLL-S6 un 8-S x 0Z -SL(-OL) un G9-GG x CE-VE lund GZ-02 x OLL-OL win/azis JOIOIA + ‘YJeEAYS snosuljeab YNOYUM ‘1 JOIOIA 4} ‘sdeo snouiejab YUM uayo saiods BunoA ‘pua Guede} auo ‘g—p(-Z) JOIOIA 4} ‘sdeo snouijejab YM spua ‘| aniq -J9|01A A|buolys +| ‘EUILUN] LUOJUd} pue ejdas PpauayO!Y} YUM “g a}dund yep 4+ ‘Huojqo ‘g L ‘uinu saiods J9]OIA ysiniq Apyeam+| winiualuAH padojanap Ajs0od ‘pasiuoqueoun ‘\UaHJAAIp 3|dloxa umoug abueio Ajjeseq ‘padojanap [Jam jou'}UaHJaAIp ajdioxa sjeysfuo Maj AJaA JO |NOYYM ‘snyjeyy snosayi6yje Aq pasaaoo Aj]e1912| ‘yoly} win QE-SL ‘aBlaq ‘auljua ajdioxa paziuoqieo Ajuly} osye Ajjeuoiseooo ed jesaze] Jamo] ‘Ayjeseq paziuoqieo ajdioxa ajdioxg eulnid UMOJg USIPpel Asapmod e yum ‘pauado Ajapim 0} AyBi|s OsIp uado osip uado Ajapim Osip abe yim uado aPIM ‘pajeedu0d Ajjeniut osip uado Ajpybijs JO pasojo osIp osiq WLU €"Q-L'0 x 0'L-Z'0 ‘peyoueiq pue snonxajj + ‘Buo| 0} ous ‘SNOJOLUNU ‘3}2]]Aull| WLU 7'0-Z2'0 x 7-G'0 ‘peyoueiq pue snonxajj + ‘Buo| ‘snoJjauunu WLU 6'Q-G'0 x E-L WWW €0-L°0 x 2-S'0 padeys -qnjo SawijaLWOS pue papunol Jo pajuiod aie yey} saoide YUM ‘ayjesed suibseyy ‘U9AOMIAIU! AjyyBis pue ‘asuap ‘pljig ‘ayeusaye ae yey} Sayouesg-qns pue sayouesq YUM JaMoleu pue Jabuo] yon ‘eau Ajasioaid eloayjode LuLu O€'0-SL'0 x €-L yBiy ww Ge0-SZ'0 ‘Ww G'0-1'0 x WU 9-1 eyeWOosSy qieX 8 ueyeunyuelins ula ewBAIOIG XI[4 3 Jableis ‘qyey (uns) Wnsnywnoaio ewBAJOIG qley 3 ueyeinuehns ‘7 asuauoyduuinyo ewbAIOIG qley asueuoosewed ewbAIOIG “*MYyoSy aUUOig eWBAIOIG Kaneas ¢°3 Aeneas 4 wnbiuiseg eubAI0Iq Jayoy ‘MV 8 Xila ‘Bupjony (xI1q) winaisejesjsne ewbAIOIG aweu saiveds ZL OL GL vL el cL LL 115 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China wun e"L-L x 7—-G"E “UOYI||!0eq ‘auijeAy elpluoo ‘E]ONSO YSIMO]|aA ayed yum SUM aulljeu} payzeagja Aj yb1s Ul pasJauuU! elpluoAd elpis! Bulaey Jey|IPEYy 8 EWES ‘0 '€ asuaye}euley ewbAIOIg = un | x G-7'9 “WUOyI|IDeq ‘auyedy ‘(jadAy -2J0ydolp!uod) Ajsnouabo1oe BIPIUOD ‘]]/eM YsImo]jaA e pue ‘WINJONSO UMOIq yep YUM ‘sem jJeLus Ul elpludAd SO]ON (6002) ‘ye 19 eflyyew (vo0z) ‘Je 19 qe (vo0z) ‘Je 19 qey (€Z0Z) ‘2 19 jooNndy (e600Z) efluyew pue euweYs (ZL0Z) Je4|IPEU pue euUUeYs (vLOz) Ie 19 ueyeinyweling (vo0z) ‘Je 19 Gey s90uaJajay (dofewu) spice olulzejes pue ‘o101NS40U ‘ONO S|AUJeW ‘ononsoidAds ‘onoIsuoo (juasqe Jo a0eJ}) ploe oNoIs ‘(e0e} JO JOUIW) plded!}91}SIOUUCD ‘Gofew) ploe o101SsJOU (juasqe JO a0eJ}) spioe ononsoddy pue ‘onons‘qns ‘ononsuosodAy ‘1101s0}dA49 ‘onoisuod ‘(Jofeww)pioe o10NS pue auoyjUeXayo)| !U jeJayduad ‘wuojunuu Je4}Ua0 = jeJayduad ‘wuojunuu SMOJ U! pabuewe AyejnBas ‘jeUua0 = jeJayduad ‘wuojunuu Je|no0| 8-S/87-6L ‘ye1]Ud9 = jeJaydied ‘UWOJNUU A|YOU Jejndo] ZL —G/9E-0Z ‘|e4UBO S jeJayduad ‘wuojunuu eydas wun /9-vE x €/Z-LVL wn 0Z-ZL x GQ-0€ un OL-SE x €0Z-SEL( -0ZL) wun /¢-EE x /VL-SOL lund Ge-ZZ x GLL-SZL lund 9€-02 x 96-0 wun (0g -)S-Sz x (SvL-)SEL -S6(-08) win/azis J9[OIA +] ‘(H-)Z-L JO[OIA +| ‘BunoA uaym ojey YUM ‘g Y9IOIA aniq +] odsopua uly} AlaA ay} AjUO JO-| JO JOJOIA +] ‘L pavsasqo jou aniq +1 ‘L JOJOIA aNIq +] ‘L JOIOIA + ‘YJeEAYS snounejab YNOYUM “1 JO[OIA +| ‘Jem alods UI} © YM ‘1 ‘uinu saiods anjg +] ong +| JO[OIA Apeam +| JO -| ong +] ong +| anjg +] JOJOIA Ayyeam +| | wuniualwiAH uoliod jeside ay} 3e Hulbsaauoo ‘aseq ay} ye Juasaid ‘saoide ye umolq ysnyoelq ‘a}el1S-p—Z AjOuUI}SIPU! ‘MO}|aA ajed ‘padojaaap A\ood a|dioxa umolq ajed 0} ysIMoj|aA ‘pasiuoqueoun Aja}8|dW09'}UabHJaAIp ajdioxa uolesiuoqued ajqewen Alene YIM ‘padojanap [Jam Ajyesa}e] pue Ajjeseq ‘\uaH-JOAIP a|dloxa umolq aBuelo Aj}OuNsIp aseq ‘\UaHJAAIp 3a|dloxa ayeuys -UOU ‘paziuoqsed-uOU ‘\UaHJBAUOD ajdIoxa umoig a6uelo ‘a|dixa au} 0} ja|jeued Jae] e WO} pue as yleq Jo syed awos ‘\UaHJAAIp a|dloxa uolesiuoqued ajqelen AJaA © UUM ‘padojanap [Jam Ajyesaze] pue Ajjeseq ‘\UaHJaAIp a|dloxa ajdioxg uado Ajayesa pou 19}2| ‘IS ‘MOWeU ‘OUYM OSIp uado = OsIpP uado osip peoiq 0} MoWJeU OSIp uado F Ajased ‘a>! -}I|S ‘MO.ueU OSIP euinid ysiym Jejnues6 e yum ‘Bunod uayM pauado AjyBis ‘uado osip uado osip osiq WLU GZ*0- LO pue Huo] Wu 9-1 aULNSS de ]foul| {WLU G0-€0xZ-L0 ‘payoueig pue snonxa}} + ‘Buojqo 0} JeAO ‘Lous ‘SnoJaluNu Uayjo ys dap e Aq pajesedas ‘sui6yew aul|jey} Hul6jnq ‘aulnssi, 9e|/OJ| ‘WW G'0 -Z0x9-L pavjasqo you elpiudAd pue eyeuoose Huo] ww G’Z-S'0 Huo] Wu Z-S'0 WwW G'0-20x0'C-7'0 ‘payouesq 0} ajduuis ‘snoJeuunu ‘9}2] |u| E}eEUUOOSe yIS daap e Aq payesedas suiBew aurjeyy Hui6ing ‘WLU 8°0-Z'0 x G-€°0 ‘peqo| Ajed ‘payouesg pue snonxalj = ‘Huo 10 Hous ‘snoJalunu eyewoosy eWWeYS 0" 8 BJeUYO ‘ellyyew lunjuabsaAuooojndioxa ewbAIOIg | SZ XI|J 8 Jableis ‘le (yosog g ‘JUOW\) wunyjasyyAla PwUBAIOIg | 7Z XII 3 Jabeis ‘gle (Bay “1nW) wnonejbida ewBbAsoIg | €Z sa190e9 ‘W3 JOONdV WuNjeIpisiojoajap ewbAIOIg | ZZ elluyew 8 BULEUS ‘O'd uunjeqjeap ewBAIOIG | LZ Jex|IPeUy 8 BUUEYS ‘O'd asualjapuep eUIBAIOIG | OZ qe g UeyeINUelINS “TF WwiNde1}3990}01du09 ewBbAIOIG | 6L X18 sableis ‘qiey (22) suanyjuoo ewBAIOIG | SL aweu saiseds 116 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China gouelsqns umouyuN ue (vLOz) Je 39 pue (a0eJ}) ploe o1011SsJOU ueyeinywehns ‘Gofeuu) pioe onons ploe olulzejes (€Z0Z) pue auoyyUeXxeyol| :O1L “-d Je 18 Jooudy ‘pad +y ‘MO]|aA +AN SNyeud ‘ploe oNdIISJOU ‘pioe d1uIZe]es ‘ploe SONS -OVL ‘MO|JaA +d ‘AHueIO +Oy ‘-O Apnis siy} ‘UMOJg USIPpad + SN|jeuy (juasqe JO 90eJ} ‘OUILW) Sploe ONONSUODOdAY (9L0z) pue ononsoddy ‘ononsuoo elr pue 17 ‘Gofeww) ploe a1}011s (vLOz) Je 32 (4oulLu) ploe o101]SUOD ueyeinywehns pue (Jofeuu) pioe o1ons {6S# 7Z0Z ‘WLUNYOS}HES# 7Z0Z ‘WiLuNYoS} (vZ07Z) joondy pue WWunyos (G68L) JINN | euoYjUeXeYol| ‘Molja +AN (vy LOZ) uewdis Sd0UdIIJ9yY ploe ononsiou Adysiwiayg Jeno0| 9—-P/LZ-SL ‘ye1]U99 = jeJaydiued ‘UWOJNUU A|YOU WJOJNW jes}uad = |e1aydisad ‘WejNDO| ZL-G x ZE-VS ‘WUOJLINUU Jejnoo|-7—-€/7 L-OL ‘ye1]U99 = jeJaydiued ‘WO JNU Je|no0| OL-€/0Z-ZLL ‘e1]Ud9 = jeJaydiued ‘UWOJWNUU A|YOU aje]das-G—Z x LL—-6 ‘WuoJUNWw BUILUN] JejNoNUa} yum ‘ajeydas-9—7 Ajassansuedy eydas wn ZE-02 x OOL-SZ wun 77-02 x 08-02 un (LL-)8S -9€(-0€) x (vLZ-)OLL -SZL( -v0L) wn gL-ZL x 09-Or wun Ge-0Z x 04-09 wun /1-SL x GG-SV wun 9-G x 0C-8L win/azis JO[OIA +] “L JOIOIA + ‘YJEaYS snoulejab INOUUM ‘7 JOJOIA +| ‘oVeY UY) YUM ‘g JOIOIA + ‘YJEaYS snounejab YNOUUM ‘1 JO[OIA +] ‘L JO[OIA -anjq Apyeam +/ deyloeq ‘g ‘uinu saiods anjq Apyeam AJaa +| J@[OlA-an|q Ajyeam +| J9JOIA ysiniq ApyeaM +| ang JEP +] JO[O!A +] | wuniuawiAH uly} AJdA ‘UMOJG -o6ues0 0} uma ‘\UdHJAAIp a|dloxa aseq SpieMo} UMOJG YSIMO]|aA ajed ‘xade ye uMOsG ‘paziuoqieoun ajdioxa uolisod jeseq au} 0} pa}o}saJ SOLUIJELUOS uoeziuoqued ‘ajyes]sqns au} jo sued ym pa|6uluuejul anssiy jeydAy ysiumoiq Jo AjsejnBa! pue Ap4eam e Jo Hulysisuos ‘padojaaap Ajueyuauipni ‘paziuoqueoun Ayjesaye| ‘\UaHJAAIp a|dIoxa uUMOJg 4Jep ‘eg aU} Jo syed uum Ajyesaye| ‘\UaHJaAIp 3|d1oxa ajdioxg pauado Ajapim 1932] ‘3>!| “Us 4Sdy. OSIP pasojo osIp eulnid ayyM © YUM palaAo0o ‘uado Ajjybis 0} MO.UeU OSIP uado osip eulnid ysiumoug Jejnues6 e YIM UMOq ‘uado AjapiM OsIp ysyuid ‘OH IFAUIS OSIP pasojo osiq Buibing your sulBew auljjeyy “WWW Z—L x GL-G"L OPIM WLU 6'Q-G" 0 x Z-€ Jo sdnoub ul payeHal66e ‘papunod ‘Aey|Os oplIM WW 70 -Z'0 pue ‘BHuo| WLW G—z ‘payoursq AyejnBa! 10 a|duuis ‘Wuaduuinia wu Z-€'0 x -L WLU L-G’0 x €-G'0 ‘peyoueiq —| pue snonxaj} = ‘Huo] 0} Woys ‘9}2 ||| E}eEUUOOSe apiM WW €'Q pue Guo} www Z 0} dn ‘payoueiq Ayyensn Anem ‘QUI|[A4I| ‘POSJOLULU! Huo wu | 0} dn ‘OpIM LULU €'0-Z'0 eyeWwoosy qiey 3 ueyeinuehns "Pp unyjaoiydxjBousaly ewbAIOIG soa0e) 'W 3 Burjany ‘joondy winsoJAB ewibAIOIG “AOU ‘ds ‘nj g'S 30M 18M asuanoyzinb ewhAsoig Bir 4Z°3 S191] ueir wnosny ewBAI0Ig qe > 3 UeyeuNUeliNs “Tr wunsouinidoosny ewbAIOIG WILUNYOS 8 JooNdy wungjuoY}UeXONSsI{ ewbAIOIG UeLUCIS "WI "H wunsua}xa eWBAIOIG aweu saiseds ce LE 0€ 62 82 LE 92 117 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China snoJauunu eps! S2]0N (LZ0Z) eyjyeyeuUIeMS (ZL0Z) Bupjon] pue eir (vo0z) ‘Je 19 Wey (vLOZ) ‘12 38 ueyeinyqueling (vLOz) 1e }8 Uls}SJENe4 (e600Z) efiuyew pue euUeYs (Szoz) 1e 18 S ueues6buoH (vo0z) ‘Je 19 qey (vo0z) ‘Je 19 qe (Z00Z) sa61e1S s90uaJajay O11 Ag payoajap (sofew) ploe oluizeyes ‘—~Ap ‘Moj|aA +d ‘—9 ‘pes yep + snyjeu} (a0e1} 10 JOUIWW) pide 91}91}SsOUU0D ‘Gofew) ploe o101SsJOU (juasqge JO 90eJ} OUI) ploe oNo1jsuod0dAuyAja0e-e ‘Gofeuw) spice ononsuooodAy pueononsodAy (JouluW) ploe onoNsodAy pue (Jofeuw) pioe olulzejes 9 Walshs JUaAjOS Ul py Jy Ajayeuurxosdde ye ‘abueso jUZDSBION|} AN UI UNO|OO a|dind jo jods e Bulwuo4 punoduwoo paljijuapiun ue: ‘Mo}a ajed +4 juasaid splioe OIUIZejes pue O1}91}SJOU pice OIJOI}SJOU ‘ploe olulzejes ‘plo® DNDNSUOD -OTL ‘MO|JOA +d ‘MOI[AA +9y ‘—O ‘UMOJG YSIPPad + SN||eY} (e0e1}) ploe dIsuauIAqns pue (4ofetuqns) pioe oWeJ} -890}0JduOd |euoIppe P9U!e}UOD UO}}99||09 9UC ‘ploeowes}900}0/d (juasqe JO 90e11}) sploe 91}01jsJOUUdDpUe ononsoddy ‘onosuo0od ‘QIUIZE|ESUOD|AUJOLUQ-F ‘onoisqns ‘ononsoydAso ‘(syseuuay Jepun aasyng ‘ofew) spioe OIJONSIOU pue O1}01S Auysiwiayo UWJOJNW Je|no0|g—-€/Z7-8L ye1]U9O = jeJaydied ‘WO JNU Je|no0| 6—-Z/07-SL ‘ye1]U99 = jeJaydiued ‘UWOJNUU A|YOU ajeidas-| €-6Z Ajassansuey Je4jUa0 = jeJayduad ‘wuojunuu Je4jUad = jedayduad ‘wuojunuu Je|no0| 8-S/07-8Z ‘azis jenba F Jo sano] auods |eujU90 pue jesayduad ‘WWOjUNUU Ajasuap Jejndo} 6-9/ZE-EZ ‘|2.41U89 = jedayduad ‘wuojunuu eydas wun gy—SE x OZL-OLL wn ZZ-91 x GLL-OZ (-09) wun Ge-Gz x 06-S9 ung x 80L-SOL win gE -V 6c x 96-62 un (0S-)Z -9€(-ZE) x (8€7-)Z0Z -0S L(-98) wun (0g -)Sv-0€ x (0SZ -)O€Z-SZL wun Gy—-0€ x (OZL ~)0S1L-S6 win/azis sued jeoide au} pue jesa}e| “sasodsoose Je JOIOIA ynNoyUM lose -onig +! JO|OIA +] ‘L -| JO[OIA +| ‘oyey uly} JoJOlA UUM ‘8-9(-p) ysiniq+| aniq JOJOIA +] ‘L ep +| —| ‘yyeays snouneyjab Aq papunoiins "UOJ “8 =| J9JOIA ani +] ‘L aniq +1 JOIOIA 4} ‘pasJadsul JIOIA +] ‘Z-L -UOU JEOIA 4] ‘sdeo snouljeja6 ym spua ‘aurljeAy ‘snose/(g—109 JOJOIA -)p-Z(-L) ysinig +1 JOJOIA +] ‘L JOIOIA +| ‘winu saliods winiualuAH uado Apybijs 0} pa}Baqu0d OsIp paziuoqueoun ‘a}a|dwu09 unjndioxa pauado Ajybijs 0} pasojo sosip paziuoqieo JOU a|dioxa pasiuoqieoun ‘\UaHJaAU0D Anybis ajdioxa uado F Ajases MOJEU OSIP padojanap Ajiood ‘pasiuoqueoun Ajje}0} ‘\UdHJBAIP 3|dIoxa uado Ajapim 0} d>{!/-UI|S OSIP pasodxa osip aseq ay} }e abuelo AjouNsIp ‘paziuoqued -UOU ‘jUaBJaAIp ‘a1elSs UOU a|dloxa uado $Sa] JO BJOW OSIP sulBieuw ua|joms ‘pasted ‘auljua Aq papunouns pue Ajjesaye] pue ‘eulnid allyM e Ajjeseq yyoq ysiumosq YUM pasaroo ‘jUaHJaAUOD JOU ‘uado apim ‘paziuoqieoun ajdioxa | = AjiejnBawul Osip pasiuoqieoun Ajje}0} ‘\UaHJBAIp a|dloxa uado osIp uonesiuoqied ajqelen AJaA eC UM ‘padojanap Ajood uado Apybiys ‘\UdHJBAIp 3a|dIoxa 0} MOJJeU OSIp ajdioxg osig Wu 90-20 x 9-80 WwW SE0-20xS 7-2 sainssl se Bulteadde de ]jaul] {WWW 90-20 xv-S'°0 WwW €0-L'0xS°C-€'0 ‘Sayouelg Ma e UiM JO ajduuis WWW 9'0-€0 x 9°L-9'0 ‘SpUd PapuNOo YUM ‘g{duuis ‘payeBuoja 0} JeyNouO Huo] WwW 9-1 payouesq Jo ajduuis Jaye ‘SnOnxa|} + ‘payeBuoyja 0} Huo|go ‘9}e]}91 ‘WW Z-G'0 x y-L a6Je| 9e} || ‘WWW L-9°0 x OL-L ‘payouesg pue snonxa|j = ‘Buo| ‘snoJalunu Uayo IS daap e Aq payesedas ‘WwW G'9 -G0x€-v0 ‘payouelg pue snonxe} + ‘Huojqo Jo Woys‘snoseuunu eyewoosy "|EUIEMS winjelpis! eWBAIOIg | Ly Bupjony 3 ele 4Z (4qiyeZ) wunuljjages! ewBAIOIg | Or XIIF 8 Jablers ‘gle WunIpauaju) BWBAIOIG | 6€ qiey 3 ueyeINUelNsS *P wunjejoadxeaul ewbAIOIg | 8E oJesel| 3 Issana4 “O'S winjejueoul ewbAI0Ig | £¢ elluye| 3 CULES (0'd gjenbaeul ewbXloig | 9€ NM M8 Bua; apy ewbfuoig | SE xI[F 8 sabieys ‘qley (1UOW\) wnonajojoy ewbA0Ig | rE qley 3 ab1e1S (‘sJad) wnolydxjbosaly ewbAIoig | €¢ aweu saisads 118 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China (€ LOZ) ‘Je 39 ELIT ap (q6002) efluyew pue euwweYs (€Z0Z) 2 19 joondy Apnis siu} Apnis siu} (S LOZ) Je Ja aqeyor/ (€Z0Z) ‘Je 19 Isuy (vo0z) ‘Je 19 qey (0z02) ula}ssone4 SNOJBLWNU BIpIs! pue joondy SO1ON sa0ualajoy pioe ononsiou “JL pai Moya +y ‘-d ‘-9 ‘_AQ eyewoose pue snjjeuy juasaid ploe ononsiou QUOYIUCX9YOI| SO1L "=d ‘-) ‘moljak +A snyyeyy pice o1dI}sJOU ‘ploe JIuIzeyes ‘plOB DNDNSUOD -OTL ‘MO}|9A +d ‘BHuelO +9y ‘-9 ‘UMOIG USIPPel + SN|[eYy} ploe oo01NsJou ‘pioe DIUIZe|es ‘plde NOMS -O TL ‘MO]|AA +d ‘MOJ|AA +O» ‘-D ‘UMOJG YSIPPal + SN|JEU} juasaid (e0es}) ploe ON0Nsuod pue ononsoydAso ‘(dofeuu) o01s ‘OL ‘-d ‘-Om ‘-9 ‘mojyjak daap +y ej|npaw pue snyjeuy sploe Oluizejes pue ONdI}SJOU :O7L “s]e}SAIO UUM MO]|aK +4 ‘AM ewoose pue snjjeu} (Jou) ploe o101sJOUU0D ‘Gofew) ploe oN0NSsJOU QUOYJUPXaYO| “OL ‘=-d ‘=O ‘moyjah +AN eloaujode pue snyjeu} Al\siwiayo aje\des-|-0/0€-0Z Je4jUad = jeJayduad ‘wuojunuu WHOJNW Je4}U90 > jesayduad 4ejnooj gL -9/07—-92 “WON jesjUuao = |e1aydisad ‘ejNDO| OL-S x 7-07 “WOJLNUU je4jUa_ = Jesayduad ‘sjjao g —€/8L-7L ‘WOJUNU Bu]U9O = jeJayduad ‘wuojunuu Jejnoo| OL —9/LE-GZ ‘Jesuad = jeJayduad ‘wuojunuu eydas lun g1-SL x GLL-OOL wun Zp-€€ x €LL-SOL wun gL-9L x 19-09 un (92-)09 -€€(-GZ) x (LvZ-)68L -ZLL( -SOL) un (vS-)9v -L@(-@Z) x (v6L-)OLL -6LL(-S6) lun GZ-SL x GOL-OS lun G*Lg -G8Lx 07Z-SZ wun Z7—-LZ xGZL -08(-09) win/azis sdeo snounejab JJEWWSs YIM Spud {yeolpuljAo Huo| ‘g JO[OIA +] ‘L JOIOIA 4+ ‘YJeEaYS snoulejab INOUUM ‘7 -I‘L oaBuelo ajed 10 JaJOIA -oniq Ajy6i|s +| ‘WUOJISN} O} plosdijja ‘Z—L J9JOIA -aniq +1 ‘8-L J@|OIA +| ‘(ZAjaued) L paasasgo you elpiudAd pue e}eWoose ‘winu saiods ong +] pasjadsul JaJOIA-on|q Ayyeam +| Ajyesaye| an|q +| Ajyy6i|s ang +] JOJOIA ysiniq Ajounsip +| winiualuAH juUaBJaAIp 0} JUaHJaAUOD a|dioxa payejnuaso JO payoeso eiqe} aseq SpiemMo} UMOJG YSIMO}]|aA ajed ‘xade ye uMO/G ‘paziuoqieoun ajdioxa ysIuUMOJq Ajyesa}e] pue Ajjeseq ‘paziuoqseoun ajdioxa jUaBJaAIp Ajaues 0} JUaHJaAUOD elqe| ‘padojaaap ||am ‘usIuUMOJg ‘paziuoqied -UOU ajdioxa aseq Spuemo} UMONq ysimojjaX ajed ‘xade e UMOIG ‘paziuoqieo -UOU‘}UaHJAU0D ‘auua ‘paonpal ajdioxa padojaaap Ajood ‘pasiuoqueoun ‘\UaHJaAIp AjyBys ajdioxa apim WW 1'9 ‘9 ‘YBly pasodxa osip uado Aj}yBi\s osip OPIM WL €°0~ ‘uado ‘je}} OSIp eulnid SUM e YUM pasaA0d ‘uado 0} MO.UeU OSIP eulnid ysimoyjaA gjed ‘uiy} e YUM pasaroo ‘uado Ajapim ‘suiBsew auljjeyy pasied aunjua Aq papunouins osip uado Apybijs 0} pajBaquod OsIp eulnid ayyM YUM ‘yor|q ysiumolq ‘pa|ea0uod OsIp apm osIp Huo} UWL 7— | ‘OpIM WWW v'0-Z'0 ‘peyoueiq Ajjensn pue seaul Huo] WwW 6 0} dn WW 70-70 x € ‘AABM ING payouelq you ‘eauly ‘Aey|OS OplM WLW 9°Q-Z'0 pue ‘6uo| Wu G’7-G'0 ‘payouesq AyejnBa! 10 ajduuis ‘Wuaduuinia oplM WLW ZZ L—-7'0 pue ‘6uo| Wu G7—-L'0 ‘seyouesq Ma} © YIM JO a|duuls ‘snonxay + Huo Wu £0-2'0 ‘peyoueiq 0} ajduuis ‘aves pasies Ajybis 0} JaAB] aWeS ‘AapIM WW 70-720 ‘buo| WWW G—Z'Q ‘aynoe sabpe ‘juadwinia 0} pasJe lu! ‘pacino 0} WYBrews WLU G'0-Z2'0 x G—-L ‘payebuoja Ajounsip oy |BAo ‘ysipunol WWW /'0-L'0 ‘payoueug AjsejnGas! 0} ajduus ‘jeopuljAd ‘snyjyeuy ay} JO Wed jejUad aU} JO SOLU HULaAOD ‘SnoJaLUNU eIpIs! ajdioxg osiq eyewoosy jooudy 3 Ssala0e9 ‘\ winsodsi6uo] ewBAI0Iq eflUxe| 3 ELEUS ‘O'€g winjejasj/6uo] ewbAIOIG sa1a0e9 'W3 Bupjany ‘joondy wunojuOYy}Uexayol] ewbAIOIG “AOU ‘ds ‘nj ‘g'S 30M 18M SnuyIUOOO] ELUBAIOIG ‘nou ‘ds ‘n4"g'S 3 nM laM asuaueysbuobis] ewbAIOIG Appey W'V 3 eyeAeN ‘aqeyoy| sisuajoousny eLUBAIOIG Jey |IPEYY "8 ELUJEYS ‘O'g asuayejewey ewbAIOIG XI[J 3 Jableis ‘ley (yosog 3 }UO/\|) nuynybun! ewbAsoig loondy LUNDIUOYZUEXBYoI/OIPIS! ewbAIOIG aweu saiseds 119 os 6v Sr Lv a or ra [Re x ” > LY (=) GV =< = = [@>) (ee) oD S oS (oa) oS vv S fy, —N wa au ev SE ~N icp) > (eB) rag se oO > S Wei Wu et al.: Diorygma from China (vo0z) ‘Je 19 qe (LLOZ) ‘|e J@ YOSquun7y (6002) ‘ye 19 ellyyew (vo0z) ‘Je 19 qe (6002) ‘ye 19 eflyyen (e600Z) efiuyew pue euuweYys (vo0z) ‘Je 19 qey SO10N sa0ualajoy (iofew) sploe 313913Su0d pueonons ‘ononsuosod hy ‘ononsodAy ploe o1N0NsJou juasald sploe o1}011S pue ‘onN91}S40U ‘911913s0}d AID (e0e}) spioe ononsodAy pue o10nssou ‘(dofewu) sploe S1ONSUOD pue 91191]suo0o|Aja0e-e ‘ONDNS (4ofew) spioe onons pue ‘o101NSs0}dA49 ‘D1JDIJSUOD ‘OIUIZE|ESUOD ‘juasaid spioe OIUIZejes pue O1}91}SJOU uawloads adh} ay} Ul puNoy Aljeuolppe ade (JOUILU) ploeoois pue (Jofeu) ploe o1913s0}dAN9 ‘(JOUILU) ploed1O1}SJOUUOD ‘Gofew) ploe o01NSsJOU Al\siuiayo Jejnoo| g—9'a}e}das Ajassansuely uunjdas jeulpnybuo| L-0 pue asJaAsuel} € UUM WwOjJuNnuugns Je4)Ua0 = jeJayduad ‘wuojunuu Jejndo0] ZL -L/LG—8Z ‘JB4usd = jeJayduad ‘wuojunuu Je4jUad = jeJayduad ‘wuojunuu Je4jUad = jeJayduad ‘wuojunuu Je4}Ua0 > jeJayduad ‘wuojunuu eydas lun G°9-S x 0Z-ZL uun /-9 xGL-CL win (7g -)E9-8E x v6Z-LSL wun GG-LZ x (0ZZ-) OLL-08 lun 9/-6S x py7- LET wun GZ-LZ x 76-92 wun ¢9-07 x O8L-SEL win/azis anjq JOJOIA +] ‘8 Ayyeam 4| aN|G-Ja]OIA +| AjBuodys ‘g JOIOIA +] ‘YJEaYS snoulejab ynoulm ‘Huojgo -LUJOJISNY “YL aniq +1 JOIOIA +] JO —| “Wem snourejab yoryy wil E-Z yum Ajued ‘SOZIS JUBJOJJIP ayinb aaey JOJOIA sai0ds ay} ‘9-7 -onjg +! JOIOIA +|‘YEaYS snoulejab JNOUYUM ‘L ‘an|g +1 JBJOIA ANIG+| ‘v—-Z | BNIq + JO[OIA (-| osje ysiniq Me} e) JJOIA +] ‘L | Apyeam 4| ‘winu sajods | winiualUAH | padojanap |jam aseq Jeindioxa ‘padojanap Ajsood Ajje193e| ‘paziuoqueoun ‘\UaHJAAIP a|d1oxa do} ay} 0} dn ulbseuw SulyJEyy 4OIU e Aq PpasaA0d ‘}UaHJaAUOD Ajyeoide ‘aurjeAy JSOW|e 0} Moja ajed ‘aseq ay} Je Juasaid ‘saoide ye umMolgq ysiyoelq ‘uonelys pOUNSIPU! Y—-E ‘padojaaap Ayood YSIMOJJaX ‘WUuaBJeAUC Ayybijs ajdioxa aseq je juasaid ‘Alyesa}e] UMOJG yep pue saaide je umolq yslyoelq ‘ayelS9-Z ‘jUaHJaAU0D ‘padojaAap A\ood a|dioxa aseq ay} ye ssajinojoo ‘uModg yep Ajjeoide ‘QELS UY} G-Z UUM ‘jUaBHJdAIP 0} jUaHJaAUOD a|dIoxa jUaBJaAlp ajdioxa ajdioxg uado ApyBbis ‘MOJJBU SIP paesodxa Ajed osip uado uayM asouinid SYM 4Oe|q O} uUMOJg Ys4oe|q ‘@PeO1g 410 9>I| LIS ‘payeaou0| ‘MOJJBU SIP uado Apybiys 0} MO.UeU OSIP MOJJEU OSIP uado A}}OuNSIp 0} MO.UWeU OSIP apim sosip osiq ys e Aq payesedas ‘QUUNSSIJ 9e]]91] ‘WW LQ x G-z ‘peyouesq pue snonxa F ‘ayeHuoja apiIM WWW GZ'0-SL'0 ‘Buo| WLU G— | peojq Wil €°Q-L'0 pue Huo] Wu 7-1 ‘Qui|ja.l| Sdueooose us e Aq payesedas Ajied ‘PASJALULUI! 9k] /9/I] WW /'0-Z0 xv —G'Q ‘SMOJ Ul Ajed Huo|go 0} jeAo ‘punod ‘snosauunu peoiq WLU GZ’O—L"O pue Huo] WU 6-S'0 ‘aul|ja.l| Sdueooose ww S'0-7'0 x €0-c20 WW J-7'0xG-L eyewoosy XI[J 3 Jableis ‘qley wuinsodsiuiw ewbAI0Ig Huron] y sasaoe9 ‘WN LunsodsoJoiw ewbAIOIG euWeYyS ‘0'E 8 JeUYO ‘ellyyew winjodsojsibaw ewbAIOIG XII 9 Jab1e1s ‘qjey winiodsebaw ewbAIOIG euUeYS ‘0'E 8 JeUYO ‘ellyyew winuwwedsebaw ewbAIOIG elUYeI\ "8 EWUEYS ‘O'g asuaindiuew ewbAI0IG XI4 8 sableys ‘qjey (ule) iobeiboew ewBbAI0IG aweu saiseds LS 9S GS + Ke) 9p) Ke) N Ke) LS 120 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China (vo0z) ‘Je 19 qe (vo0z) ‘Je 19 qe (vLOz) Ie }9 UlsySJense (6002) ‘Je 19 ellyyen (0z0Z) yBuis pue ybuls (€Z02) ‘Je 12 ooNdy (€Loz) Bupjom pue eje|d wun g*L-L x p—-€ ‘WuUOyIOeq “auljeAy elpiuoo :LUNJONSO JeyJep e ULM SLIEM aulj/ey} |Jeus Ul pasJaLULU! (v00z) elpluoAd ‘Je 38 qIey S3]0N SODUDIIJOY esjUad = |e1aydisad ‘4e|ND0| OL-9/(S7 ploe ouesja00}01d -)G€-SZ ‘WOJUNW (juasqge JO 90eJ} OUI) Sploe O1}011S puke 91}01}SUOD ‘onosuosodAuyAja0e-e ‘Gofetu)spioe o101suod0dAy Jejnoo|-7-€/ZL-ZL ‘ye1]UdO = jeJaydied pue ononsoddAy ‘UWOJLINU sploe 91}01]SJOUUOD puke J1}01}S10U “OTL ‘UO!OaS DIdodSOJOIUU Ul sjeysAuo padeys-a|paau ‘pal ayeydes-6-/ -oBuelo Buiseajas ‘Moja +4 Ajassaasuedy juasald sploe 91391;S|AyjauU jeyuao = pue ‘oluizejes ‘ononsjou jeJayduad ‘wuojunuu sploe o101suoo pue ayeydas-/-G 91]9SJOU ‘QUOYJUPXaYO!| Ajasieasuedy $9|NDO] 9 O} dn yym Wuojunuuqns 0} a1e1das-¢- | ploe ONONSJOU ‘OTL “-d ‘pad < Mo}|aK + ‘-AN snyeUt (saoeJ} JO JOUILU) sploe olonsuooodAy pue ‘ononsoddy ‘ononsoydAo ‘(ofeww) sploe a1ONSUOD pue onons ‘(mo|jeA+A\N eens snjjeuy) auoYyUeXaYo)| WuoJUNuY A]YOU (doretuqns Jo JoleW) spice onodNsuosodAuyjAja0e pue ononsuosodhy ‘ononsodAy Je4}Ua0 > jeJayduad ‘wuojunuu Aasiwiay9 eydas un gs -SZ(-6L) x (OZL -)0S1L-S6 wn gL-OL x G9-0r un / x CE-8C wn 0g-7Z x 66-SZ win g-7 x 8Z-8L wun g-¢ x 1-9 wn O€-02 x OZL-08 un 09-GeEx GOL-SOL win/azis J9JOIA +] ‘(Z-)L J9/OIA +] ‘8—9(-1) JO[OIA -oniq +| ‘yyeays snounejab Aq papunouins ‘g J9[OIA +] 'T-L aniq +1 ‘8-9 JOIOIA + ‘UJEaYS snouljejab NOU ‘8 “ANIG-OIOIA +] ‘L JOIOIA +] ‘(Z-)L ‘uinu saiods AjyOUuNSIp +] | wuniuawiAH | JO[OIA ysin|q UsIUMOJg JO pasiuoqieo Apejnbau! Ajyeam +4| ‘\UaHJBAIp a|dIoxa pasiuoqueoun Ajyeyoy'yuahsaAuoco Anybijs ajdioxe JOJOIA Ysinig +] yuabieaAuoo ‘saoide ye uMolq ysryoe|q ‘Ajje10}2| uMoJq ajed ‘uly} ‘aseq ay} Je JUasaJd ‘a, els €-Z ‘padojanap an|q +! Ajsood ajdioxa ulBseu auly/euy jesaze] Aq paiaaoo -| ‘paziuoqieoun ajdioxa ‘paziuoqieoun Ajaya|duuo09 wunindioxe payesouabap Aq ayeuys Ajyed ‘umolg AaJ6 Ajje1912} ‘padojanap AjJood A\jeseq ‘pasiuoqieoun yuaBiaauoo AjUBis Jo jUaHJaAIp a|dioxa J9JOIA ajdioxg X9AU09 F Ajased ‘uado osip ‘uado F Ajaues ‘MOdJeU OSIp pasodxa osip asouinid uado UaYM ‘MOU SSID pasodxa 0} MO.UeU OSIP api WW GQ'0~ ‘P910]09-Ysal} OSIP asouinid -UOU ‘YOe|G-UMOJG ‘pasodxa osip uado osip osiq ys daap e Aq payeiedas ualjo ade SOsiIp ‘auuNnssij EAI] {WU L-EOxGE-L ys deap e Aq payesedas uayo ‘WWW v0-20xv-S°0 WLU LQ x L°L-€ “0 WLU €*L—-G'0 pue Huo] wu Z| payouegq 0} a|duuis ‘pasJoWU! ‘a}e ||| E}eEUOOSe LWIPIP Ul LULU G 0} dn ‘apim WW 7 O~ ‘payouesq Ajpayeada. ynq ‘reaul| ‘sdnos6 SOO] U! JO Aley]OsS SpIM WW 70-70 ‘Huo| WLU E-G'9 ys e Aq payeiedas uayJo ‘WWW G‘0-v'0 xS-S'0 eyeWwoosy X14 sabiers ‘gle (Myos3) winsouinid ewBbAs0Ig | S9 XIIF 8 Jablers ‘qjey (894) faeyod ewBfi01g | 79 sa190e9 ‘W3 OONdV ‘eYUND ‘Yd’ | “Jssane “JS wunjejdasioned ewbAI0Ilg | €9 eWWWeYS 'O'E 8 BJeUYO ‘ellyyew asuaiuebyoued ewbAI0Ig | Z9 yBuls d 33 yBuls idysnd (efluye 3 “mepy) winjjnoo0 ewBbAIOIg | 19 sa1a0e) 'W3 Burjony ‘joondy OULOJLNWIGNsJOU ewbAI0Ig | 09 Huron] ge e}e|d SeAY sueoubiu ewBAI0Ig | 6S XI[J 8 Jableis ‘qiey (‘|AN) winsoydouow eWBAIOIG | 8S aweu saisads 121 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China wun g*L—| x G-77'9 ‘WUOyI||IDeq ‘aueAy ‘snouaboioe PALO} eIPIuCD ‘Jem aHuedso uly} pue Lunjoso uMmoug 0} abueso JOUN}SIP YUM ‘padeys-sead ‘SHIEM ||BLUS Ul pasJeWUU! ‘SnoJeuunu (v00z) sploe elpluoAd ‘je 1a qey olulzeyes pue o190Ns10uU (e0es}) ploe oNONSu0U pue (JoulwW) poe ononsed ‘GOuILU) ploe 919013S0}d AN (yLOz) 1€38 =| ‘(4ofewiqns) pioe aiulzejes ueyeinywehns ‘Gofeuu) ploe o1NoNs (Mo}|a4 +) (ZLOZ) juasasd (JOUILW) 91O1}S0U Je]IPEYY pue (Jofew |j2) 130s pue pue ePUueUS ‘ononsoidAds ‘onoNsuoo (46002) efiuyew juasaid spioe (Jofew) pue ewweys | ojos pue (“ofew) 91}91]su09 (e0eu}) sploe olulzejyesuod pue ononssouqns ‘(uawloads ad} ay} ul Huryoe] poe oluizeyes yng Yofew (v00z) Ajjensn) spioe oluizejes pue ‘je ja qey 9121}890} 01d ‘O1}91]4Ss10U ajdund +y juawuBid ‘sjeysA490 pedeys-a|paeu ‘pas Buluoy XNIJJ@ MO]JAA + YUM UO!}OaS dIdoodsoJolwW ‘pasaBbueso +d snjjeu} ‘eyeuuoose (7 LOZ) au}. Uo JUaLUBId par-yuld ‘}e 1a Buodeqg | UMOUUN pue pide ONONSJOU sploe oluizeyes pue 921}990} 01d ‘auOUUeXaYo!| SUIEJUOD UOIDa]]09 BUD ‘(a0eN)sploe a!uIZejesuod pue o101}sJouqns ‘(“Wofew) (v00Z) sploe ojulzejes pue ‘je ya qrey 921}890} 01d ‘91}91}S10U Sd]ON Sd0UdIIJ9Y Alysiwiayg Je4]Ua0 > jeJayduad ‘wuojunuu Je|Nd0| OL-S/87-SZ ‘e1]U99 = jeJaydiued ‘UWOJWNUU A|YOU SMOJ UI pabuewe Ajsen6as ‘je.uac = jeJayduad ‘wuojunuu Je4jUad = jedayduad ‘wuojunuu Je4}Ua0 > jeJayduad ‘wuojunuu euluun| sejnbue YIM 91e]daso}sipqns ‘WJOyLNnw AjYyou Je4}Ua0 > jeJayduad ‘wuojunuu eydas lun G/-0¢ x (0&7 -)00Z-OSL | JJOIN +] 40 -] ‘1 wun Gy—-0€ x GVL-OOL JS[OIA +] ‘L wun Z7-SZ x /yL-88 un 96 -17G x GOZ-89 L( -SEL) J9[OIA ON|Q+] ‘T—L JO|OIA +| ‘YYEaYs 4O!Y} wu G*7—G YIM ‘1 wun (¢9 -)Sv-0€ x (GLZ -)SSL-OZL | JOJOIA ani +] ‘L wun 0€-02 x 00L-09 L wun gg-GE x O€Z-OLL win/azis J9JOIA +] JO -| ‘L ‘uinu saiods JO[OIA Apyeam +| 10 -| ong +] ong +] ysiniq Apyeam +| JO -| JO[OIA Apyeam +| winiualuiAH ajqewen uonesiuoqies ‘Alyeoide ‘aseq au} 1 pasiuoqued 0} umog-e6uelo ‘\UaHJAAIp 3a|d1oxa uado osip eulnid ysiumoug ajed 0} anym Alapmod O14} YIM ‘pauado Ulu} AJdA ‘UMOJG -oBues0 0} umalg 1461 ‘uaHJaaip ajdixe Ajapim osip sdij ay} ye paziuoqueo ‘ayes Z| Ayjeoide uado F ‘\uaHaAUOD ajdioxa =| Ajaued ‘MOWeU OSI asouinid ‘apim ‘JI ‘USIUMOIG O} jUabJaAIp ajdioxa YsIMo]|aA OSIp Ayeyuauuipni ‘pasiuoqueoun Ajyey0} ‘JUaHJaAIp }e]J ‘MowweU Ajjounsip a|dioxa JO apIM OSIP eulnid pas-yuid Jo sayoyed yUM pasnjjns asouinid -SUYM ‘UMOJG ‘MOJJEU SIP pasiuoqieo Jo ysiumodg Ajje18}e| pue Ajjeseq ‘juabaaIp Ajjounsip 3jdioxa "JE]J APIM OSIP ajdioxy osig aseq ay} }€ Pa}OUJSUOD ‘LULU 80-70 x OL-L WwW L-SO*xL5-60 azis ‘payouesg pue snonxa|} = ‘Huo 0} Hous ‘snoJauunu ‘a}2] |u| E}EUOOSe payoueiq AyejnBa.u 10 ‘ajduuis ‘Buo| WLU G'Q-Z'0 payouesq ‘juaBawWa ‘Buo| WLU e— | 3NO}s 9e]/9J1) ‘UW 8'0-17'0 x v—L ‘Bbuojgo 40 |BAO-ysipunol yBiy wi Z'0-SL'0 ‘pecq WW 7'0-€'0 ‘buo| uwulW /—¢ ‘payouelq Aja,e]|a}S S9SEq Pd}dIJ]SUOD uum Aled ‘ajissas ‘INO}S 9e]] AJ!) “UW L-€'0 x 7-G'0 ‘Buo|qgo JO jeAoO eyewoosy XIIF 9 Jableis ‘gle asuaopeajes ewBAJOIG qe g UeyeINelins “Tt wngiuizejes eWBAIOIG eflyyeW 8 ELUEYS ‘0'G ejooldns ewBAI0IG efluye 8 BUUEYS ‘O'd (IN “YO 8 MEd) wunsodsoyns ewbAIOIG X14 8 sabes ‘qiey Geyouy ‘M'V) winsouinidojnd ewbAIOIG uae, 9 Buryom ‘Buoded winjeuinsdoasol ewbAIOIG XI[48 sable ‘gle (224) auUojiuas EWBAIOIG aweu saiveds cL LZ OZ 69 oe) Ke) N Xe) 99 122 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China (4oulLu) ploe oves}900}0/d pue UUW (JouIW) ploe o1}01sedioU 7 L-Z'0 ‘PeHojsip ‘GdoulLW) ploe oluIzejes SOLUIJ@LUOS (ZLOZ) ‘GOuILU) ploe ONONSJOU wn OV-Zé ‘paziuoqueo yejnoulo Ayensn X19 3 JOU “MV XIJq pue JaYOY ‘Gofetu) auod}OaU UWJOJUNUWW x O9L-OZL an|q +1 ‘L -| —uUOU a] dIoxa ‘Q|ISSAS ‘WUOJIOSIP | suUeLUIaS eEWBAIOIG | 08 BUILUN| papuno pue ejdas paudyo!U} YUM ‘wunjdas jeulpnybuo| yBbiy Bupjom (LLOZ) sploe ononsoydAso L-0 pue asJeAsued} un g-9 aN|G-}9[OIA pasodxa Ajjed WW LO ‘Wwwe'g | Bagley ‘uUeyYeunyQUelins ‘Je 8 yosquuny pue ‘ononsoddy ‘o1j01s © UUM WWJOJUNLUgns x €L-OL A\Buoys +] ‘8 0} payeaouod OsIp -GLOXxE€-L wnogioNns eWHAIOIG | 6/ yoerlq ysiumoq wun Gy—-0€ ‘PASJOLULU! YyBuls dy (Z LOZ) ybuis jes]Ua0 = x (OVL JOJOIA anjq jUaHJaAIp ‘a|duuis ‘wuoyijeul| | 9g yBuls idysnd (UNS) pue yBuls ploe oI01}SJOU jesouduad ‘wuojunw = -)OLL-06 | Ajjurey +) JO -| ‘L +] 28M | ‘paziuoqueoun ajdioxa pasodxa osip eloaujode winjojids ewbA0Ig | 8/ (4ouluu) auoYyjUeXayo}| ‘(adeJ} JO JOUILW) ploe 91}91}Ss1OUU0D ‘Gofew) ploe o01NSsJOU ‘MOJ|aK ajed +A ‘ebueso peoigq +d ‘sje}sAlo pedeys-ajpaau YSIMo]jaA MOJJEU JaY}eI WW 9°0-17'0 ‘Buo| (6L0Z) ‘pas BulLudoy ‘xn]JJa Moja un Gp-seE an|q 0} paziuoqieo ‘pasodxa Ajued | wil G—| ‘payoueiq Huron] 3 ew 74 ‘je Ja PUI ap 1 YUM UO!O—aS U! SNyjeuy wwojnuu AjYyou XOVL-OZL | -J9/OIA +] ‘Z-L SSd|JO]O9D Ajjesaye] wunjndioxa =| 0} payeaou0d OsIp AyrenBa.d! winuelydos ewBbXl0ig | // sploe Je|Ndoj -8 JOJOIA padojanap ‘MIUSey (v00Z) 919IJsJOUUOD puke ONONSIOU | —//OE-O0Z ‘JeNUeo= wNnsGp—sE ysin|q A\iood ‘pasiuoqueoun aPIM UO J9}e] 9 ‘UBYEN ‘W (q|4eZ) je 1a qey ‘Pas 0} MOIJAA +d ‘Pal +y jedayduad ‘wuojunuu | x OVL-OLL JOJOIA 4] ‘L Ajyeam 4| ‘\UaHJaAIp 3a|dloxa ‘MOJEU JSJIJ OSIP | WLU 9°0-7'0 x G-L winuezoos ewBbX0Ig | 9/ J@JOIA YSIppal padojanap IS daap Ajyeam Ajaied Ajsood Ajjes93e] ‘UMOIG e Aq payesedas (juasqe JO JOUIWW) +| 40 —| ‘BunoA 0} aBues0-YsIMoj|a JO Ud}JO ‘PaSJOLULU! ploe oNo1jsuod0dAuAja0e-e Je|noo| wun (Ov uaym aodsida JO|OIA Ayeyuauuipni Ajjeseq de ][uly ‘WWW (v00Z) ‘Gofew)spioe oasuosodky | g-G/OE-vL ‘Teued= -)OE-pyL | snounejeb yo1y) ‘juaBienlp AjyBi\s 10 uado F 0} v'0-20xv-S'0 XI[J 3 Jableys ‘grey ‘Je Ja qey pue ononsoddAy jeJayduad ‘wuojunw | xQOL-Sp | wing e uy ‘9-Z ang +! jUaHJaAUOD ajdioxa =| MOU UaYO OsIp | ‘ayeHuocja 0} WOYS nuewidis ewbAI0Ig | SL quaBiawa A}}4y61\s 0} pasJaluu! ‘paBueuwe Ajasojo Aja ‘payouesg 0} ajduuis ‘ayeHuoja 0} puno ‘snonxajJ SSd| JO BJOW ‘WOYs (q6002) juasaid peoiqwweg | ‘parino ‘snoJeuunu efiuyew sploe (80eJ}) O1011SJOU jes]Ua0 = un vE-6Z JOJOIA paziuoqeo-uou -Z'0 ‘peolg 0} ‘SnoJO|ONUOD efluyyeW 3 euUeYS pue ePLUueUS pue 91}91}SUOd ‘OIONS jejayduad ‘wuojunu | x ZZL-€rL JOJOIA +] ‘Q-| ang +! ‘\UaHJAAIp 3|dloxa MOU OSIP sdieo0ose ‘O'€ ejooixes ewBAso0Ig | vy Huo] Wu soa0e) ploe olulzeyjes JOIOIA 4 ‘YJeaYS L 0} dn ‘apim Wu ‘W 3 Buryjon ‘Joody (€Z0Z) pue auoyjUeXayol] :O1L “-d un ZE-6Z snounejab G'0-€'0 ‘peyouesq LungjuOYUeX/es ‘Je 19 Joody ‘pad +y ‘MO]JaA +AN SNyjeuy UWWJOJUNUWU x OVL-OEL yNOYUM ‘1 “pasojo osIp }OU ‘eaul| ‘Aey|OS ewbAoIg | €Z S210N So0UdJojay Adysiwiay9 eydas winl/azis ‘winusaiods =| winiuawAyH ajdioxg osig e}yeWOOSY aweu saisads 123 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China (vLOz) 1e }8 Uls}SJENs 4 (vZ8L) Jajlamyosy ‘un | x P-E UOJ |IOeq ‘auijeAy elpluoo ‘WUN|ONSO ysiuMolg e UM SEM Oul|/EY} ||eWUs Ul pasJeLuLU! (vo0z) elpluoAd je 1a qey (vz0Z) ‘Ie 38 IND (vLOZ) 1e 39 ueyeinyywelins Jayoiy ‘M’V Sisuasi/e} (L002 eulydesy = ‘E00Z) 4e4UdI'Y (vL0Z) ‘Ie 38 ueyeinyqweling (46002) ellen pue euwweYys Sd10N S80UdIBJOY ploe oWe1}900}01d “LL ‘aBuei0+_ ‘MO||aA daap +4 snyjeuy sploe o101)SJOU pue oluizeyes-uoojAuyau 0-€ ‘OluIzeyesuod ‘OIUIZe|eS “pioe onjonsoddy jo $99} JUICE} SALUSLUOS ‘(s}unowe snoueA ul) sploe onoNsoydAio pue o101Suo0o ‘ononsaqns ‘ononsiued ‘(lofeuu) ploe o0Ns ploe oNoNsJou ‘Pal 0} MO}JAA +d ‘Pal +y (4OuILU) ploe 91301jsUOD pue (Joutuu) ploe onONSoydAN9 ‘(lofeu) ploe onoNs ‘Gofeu) ploe ouesa00}01d ploe onjoys (4oulLU) pioe onons|AyAW pue (iofewiqns) pice anjonsodAy ‘Gofeuu) ploe suesja00}01d juaseld sploe onols pue ‘oNoNsJOU Alysiulayo WJOJNW Jejndo} 8—/07-S L"WuOjNwW JeujUad = jeJayduad ‘wuojunuu Je|n30| S—7/6L—-ZL ‘e1]U99 = jeJaydied ‘WWOJNUU A|YOU UWJOJNW Jejnoo| G—/ZL—-9L e1]U99 = jeJaydied ‘WWOJINUU A|YOU JeujUad = jeJayduad ‘wuojunuu eydas wun G{L—-OL x OV-17Z un og -02(-91) x 06-SS wun Q9-GE x OLZ-OZL wn ZE-/Z x GOL-Z8 wun py-0€ x 9EL -00L(-06) wun Og-SZ x 06-08 wun ye-7Z x Gv L-SZ win/azis aniq Ajurey +] ‘uzeays snounejab APIM LULU G “Oo Aq papunouns ‘spua papunol UUM WUJOJISny Ajpeoiq ‘aujedy ‘g -| suoljejnuue 9jdiynuu ym ‘{EOLIPUI|AD-8}2A0 ‘aBue] aedaU} =| Bujuayo!y} [JEM ‘J9[OIA + ‘BunoA uayM jsea] 1e JO[OIA ‘(wun ¢-L) Jem | Apyeam 4| ajods yoru, YUM =| = Ajjesaye| uayo ‘y—Z(|) Jo -| —|‘oyey UIUY YUM *L =| aniq Apyeam t]‘L | 8IOlA 4| aniq +1‘L JOIOIA 4 ‘YZEaYS snourjejab e JOJOIA NOYWM ‘ZL Ajeam+| JO[OIA J9/OIA +] ‘B—L aniq +1 ‘winu salods | winiualuAH | padojaaap Ajiood ‘pasiuoqueoun Ajje}0} ‘\UaHJBAIp a|dIoxa Ja}e| ‘\UaHJBAIP a|dloxa ‘asieds SAWIeWOS pasiuoqied 0} UMOIq ylep‘juabaip a|dioxa pasiuoqueoun a|dioxa sedoid ayelys AjJOUNSsIpU! ‘paziuoqued-uou ‘WUaBIdAIP 0} jUaHJaAUOD ajdioxa ajdioxg pasodxa Ajmoueu OsIp (Saseo aLUOS UI JojO9 ul YsIppea,) pajauueyo pue 3e|} S| OSIp ‘uado syds uado osIp uado osip uado Ajapim Osip uado Bulwiosaq SOLUIJBWOS ‘paso|o sdl pauado Ajapim osip peoiq 0} MoWJeU OSIp osiq ayeoloo you ‘ainua sulbew ‘WW O'L x 0°9-S'0 Ajjenjuane jey} snyjey} e ulyM pasojoua ‘payouelq yeEYMaWOS ‘ayeBuoja Ajseaul| pue Buojgo eloayjode WILL 9°Q—-€'0 x G—G'0 ‘payouesq pue snonxa ‘Buo] ‘snoseuunu uayo sduesoose WLW Z-7'0 x €-G'0 WLW O'L—-7'0 x 0'€-S'0 ‘(seyoueiq M2} & YUM) ajduuis ‘snonxal} F ‘HBuojgo 0} jeao ‘yey Aounsip ‘SnoJjeuunu WW 7'0-Z'0 x €-G' | ‘snonuls JO PaAino ‘pajayeos WWW élL-v'0 x €-7'0 ‘payouesq- | pue snonxa|} = ‘Huo 0} Hous ‘snoJalunu eyewoose LULU 9°Q—-€'0 x G’7—-S'0 ‘peyoueiq ‘SNONXa|} 0} paaino 0} Ybies eyewoosy joondy 3 eyUND ‘Yd “}suene ‘9S asuasuUezO} ewbAIOIg | 88 “MYyoSy wunuojoul ewHAOIg | £8 XIIq 3 Jable1s ‘qlem mjequ ewBA0lg | 98 elt AZ asua/ejuel} eWBAIOIG | Sg qie 8 ueyeunyuelins wungipueyley) eWwBAIOIg | 78 Jay ‘MV (124 ‘M'V) sisuasije} ewBhAIOIg | €8 qley 3 ueyeINuehNs ‘7 winsouinidqns ewbAI0Ig | Z8 efiuyeW ge euueUS ‘O'€ (elle 8 Mied) wunjeqgjeqns ewbAsoIq | 18 aweu saiseds 124 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China “By ‘IN winueiuosjim siydei9 = pioe 91101]S10U pue QUOUJUPXYOI| Jo aouasald ayy Aq pazuayoeseyo S210N (S00Z) 4e4QIy (g002) XI|q pue Jayouy Apnis siu} (e600Z) elle pue euUUeYS (vo0z) ‘Je 19 qe (€Z0Z) 2 Ja joondy s90uaJajay (Jouluu) ploe oNONSLad pue (Jofeuu) pioe onons ploe SHONSJOU SOUL ‘MO]|AA +d ‘MOA +O» ‘-D ‘UMOJg YSIPpad +y SN|jeu} juasaid sploe olulzeyes pue o1}91)SJOU spioe 91J011SJOUUOD puke dIONssOU ploe 91}91]SJOU pue BUOYJUeXaYOI| ‘OL ‘-d ‘Pal < Moy|aA +> ‘Mojjak +N snyyeur Adsiwiayg wirl6-8 9}€[NDO|-9L—-OL x 0S-Sr WJOJISNY ‘g JBJOIA Jejnd0/-ZL-OL win QL-8 | -an|q +] ‘eJelas-Z Ajassaasuedy x 0S-OV ‘snose Jad g un (Ly-)Se€ Jesju80 —8L(-LL) x = Jexaydiied ‘sejndo| 6 | (Z7L-)OLL —9/87-v ‘Wuoj”nW | —E€/(-Sg) JOJOIA +] ‘L jejuao = unl ¢€¢-SGZ jedayduad ‘uuojunu | x 9ZL-/6 anig +1 ‘L jeyuac > win gy-rr | “Ja[OIA +] Apyeam jeJayduad ‘wuojunuu | x OSL-OOL Ajuo JO -| ‘L paAjasqo jou eydas winl/azis ‘uinu saiods JOJOIA + ang +] JOJOIA ysin|q ApyeaM +| | wuiniuawiAH | aunjonijs jeseq 2 Buryoe| ‘UIU} Ayan ‘yor|g-uMolq sep wunioayyied yoelgq ‘uado Ajapim ‘ely OSIp asoulnida UMOJG-UsIPpaL apiM Ww ‘MOWEU B [2aA21 0} | €0-Z'0 x (S-)p-L jOUI}SIpU! ‘peziuoqued | BHuluado ‘pasojo | ‘payoueg ‘snonuis -UOU ajdioxa Ayyeniur sdiy Jo panino ‘yybies OpiM WW 70-10 pue ‘Buo| www /-Z ‘aynoe JO papunol Ajjeulue} aseq SpieMo} eulnid Jaya pue UMOJg YSIMO]|aA SUYM UI4}e YUM | ‘padino ‘payouesq ajed ‘xede ye uMO/G P249AO9 ‘dyII-HI|S Aja,e]|a}S ‘paziuoqseoun ajdioxa | 0} pasojo Osip ‘yuauIWuO1d umolq abueo AjOulsIp aseq ‘paziuoqieoun | apIM WW ‘0-20 asniqo ‘jUaBIAAIp ‘sje}SAJO ‘uayuns ‘apIM 0} 8]nde spua Aq pasaaoo ajdioxa 0} MOJJEU OSIpP ‘Buo] WLW ZL Bulbing ‘WW L-'0 x padojanap Ajood ‘pasiuoqueoun ‘\UaHJAAIp 3|dloxa G-— L‘peyoueiq pue snonxe} + ‘buojqo 0} JeAO ‘ysipunod @PIM UO J9}e| ‘MOJJEU }SJIJ SOSIP parlasqo }OU eyeWOose ajdioxg osig eyeWOosSY Jayouy MV (Bay “1nW) winueluosim ewbAs0Ig XI[F 8 JOUoY ‘M'V Sisuauewel//em ewbAIOIG ‘aou ‘ds ‘n4°g’S 3 NM 18M am ewBAOIG elle 3 ELUeYS ‘O'g WNsOWUIONIEA ewbAOIG xI[J 8 Jableis ‘qiey (ung) wunsojnosagny ewbA0IG ‘$ala0e9 ‘3 jooNdy winuelbiaqsuac} ewbAIOIG aweu saiveds v6 €6 c6 L6 06 68 125 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 Wei Wu et al.: Diorygma from China ethanol (P). Lichen substances were analyzed by thin-layer chromatography (TLC) using the solvent C system (formic acid/acetic acid, v/v = 200/30) (Cul- berson 1972). DNA extraction, PCR amplification, and sequencing Genomic DNA was directly extracted from apothecia using a fungal genomic DNA extraction kit (Solarbio, China), following the manufacturer’s protocol. Mi- tochondrial small subunit rRNA (mtSSU) sequences were amplified using the primer pairs mrSSU1/mrSSU3R, while large subunit ribosomal DNA (LSU) se- quences were amplified using the primer pairs LR5/LROR and AL2R/LR6 (Vil- galys and Hester 1990; Zoller et al. 1999; Mangold et al. 2008; Kraichak et al. 2015). Polymerase chain reaction (PCR) was performed using a Bio-RAD T-100 thermal cycler in 25 uL reaction volumes, consisting of 12.5 uL of 2x PCR Mix (including dNTPs mix, Solarbio, China), 8 pL of double-distilled water (ddH,0), 1.0 uL of each 10 mM primer, and 2.5 pL of DNA template. The PCR conditions were as follows: an initial denaturation at 95 °C for 5 minutes, followed by 38 cycles of denaturation at 94 °C for 45 seconds, annealing at 50 °C for 60 sec- onds (for mtSSU) or 55 °C for 60 seconds (for LSU), and extension at 72 °C for 90 seconds, with a final extension at 72 °C for 10 minutes and held at 12 °C (Kraichak et al. 2015). The PCR products were visualized by 1% agarose gel electrophoresis and subsequently sequenced by Beijing Tsingke Biotech Co., Ltd. (Chongqing, China). Phylogenetic analyses The sequencing results were evaluated by analyzing chromatograms using BioEdit Sequence Alignment software (version 7.0.9.0). Forward and reverse sequences were assembled using ContigExpress software (version 6.0.620.0). Preliminary taxonomic affiliation and potential sample contamination were confirmed by BLASTn searches on the NCBI website (https://blast.ncbi.nIm. nih.gov/Blast.cgi). Newly generated sequences were deposited in GenBank (Table 1). Additional sequences used for ingroup analysis were retrieved from the NCBI website (https://www.ncbi.nim.nih.gov), and Glyphis cicatricosa was selected as the outgroup (Ansil et al. 2023). Phylogenetic analyses were con- ducted using the OFPT program (Zeng et al. 2023), following its protocol: each gene region dataset was initially aligned using the ‘auto’ strategy (based on data size) in MAFFT (Katoh and Standley 2013) and subsequently trimmed us- ing the ‘gappyout’ method (based on gap distribution) in TrimAl (Capella-Guti- érrez et al. 2009). Single-gene phylogenetic trees were constructed using the IQ-TREE Web Server (http://iqtree.cibiv.univie.ac.at/) to confirm well-supported branches. The best-fit nucleotide substitution models for each dataset were selected based on the Bayesian Information Criterion (BIC) from 22 common DNA substitution models with rate heterogeneity, using ModelFinder (Kalyaana- moorthy et al. 2017). All datasets were concatenated with partition information for subsequent phylogenetic analyses. Maximum likelihood (ML) analysis was performed using ultrafast bootstrap approximation (Hoang et al. 2018), com- bined with the SH-like approximate likelihood ratio test (SH-aLRT) (Guindon et al. 2010) in IQ-TREE (Nguyen et al. 2015). The consensus tree was summarized MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 126 Wei Wu et al.: Diorygma from China based on the extended majority rule. Additionally, ML analysis was conducted using RAXML-HPC2 on ACCESS (version 8.2.12) in the CIPRES Science Gate- way (https://www.phylo.org/portal2/login!input.action) with the GTRGAMMA model and a rapid bootstrap analysis of 1000 replicates (Miller et al. 2010; Sta- matakis 2014). Bayesian inference was performed using MrBayes (Ronquist et al. 2012), with two parallel Metropolis-coupled Markov Chain Monte Carlo runs (one ‘cold’ chain and three heated chains), sampling trees every 1000 generations. The run was automatically terminated when the average standard deviation of split frequencies dropped below 0.01, and the resulting tree was summarized after discarding the first 25% of samples as burn-in. The resulting trees were visualized in FigTree v1.4.4 and further edited in Adobe Illustrator CC 2019. The new taxon was registered in Index Fungorum (2025) and Faces of Fungi (Jayasiri et al. 2015). Result Phylogenetic analyses The final dataset comprised 22 taxa and 39 strains/vouchers, with 1562 aligned characters including gaps (LSU: 760 bp; mtSSU: 802 bp). The RAxML tree was constructed with a final ML optimization likelihood value of -6039.173664. The parameters for the GTR+I+G model of combined LSU and mtSSU were as fol- lows: estimated base frequencies—A = 0.29, C = 0.19, G = 0.27, T = 0.25; substi- tution rates—AC = 0.66, AG = 2.55, AT = 1.69, CG = 0.76, CT = 7.83, and GT = 1.00. Bayesian posterior probabilities from MCMC analysis showed a final average standard deviation of split frequencies of 0.009999. The topologies from both ML and Bayesian analyses were verified manually and largely concurred (Fig. 1). Fourteen known species of Diorygma formed a well-supported clade in the phylogenetic tree. Three new species-—D. leigongshanense, D. locitonitrus, and D. guizhouense-clustered in a clade with D. tiantaiense Z.F. Jia, with strong support. In contrast, Diorygma weil was related to D. karnatakense, but this rela- tionship received low support. Taxonomy Diorygma guizhouense Wei Wu & S.B. Fu, sp. nov. Index Fungorum: IF903744 Facesoffungi Number: FoF 17083 Fig. 2 Etymology. The specific epithet “guizhouense’” refers to the location where the holotype was collected. Holotype. KUN-L 0093724 Description. Sexual morph: Thallus corticolous, crustose, thin, tightly at- tached to the substratum, pale grey to greenish grey, rough, dull, lacking isidia and soredia, prothallus absent. Apothecia lirelliform, scattered or aggregated, erumpent, simple or irregularly branched, curved, and either terminally round- ed or acute, measuring 2-5 mm long and 0.2-0.4 mm wide. Disc narrow to slightly open, covered with a white pruina. Exciple uncarbonized, brown at MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 127 Wei Wu et al.: Diorygma from China Diorygma karnatakense 21. 52 AMH Diorygma karnatakense 21.55 AMH 100/100! Diorygma karnatakense 21.26 AMH Diorygma karnatakense 21. 54 AMH Diorygma karnatakense 21.60 AMH Diorygma weii L 0093727! KUN 95/0.91-— Diorygma leigongshanense L 0093725" KUN 100/0,29 | Diorygma locitonitrus L 0093723" KUN 2B/L00} =| Diorygma tiantaiense Jia ZJ19123" LCUF Diorygma guizhouense L 0093724" KUN Diorygma hieroglyphicum Wirth 26647 Diorygma sp. Lumbsch 205011 F 6 /., Diorygma antillarum Liicking 33018 F 78/-|- Diorygma antillarum Liicking 33019 F 99/100 | Diorygma antillarum Nelsen 4185 F Diorygma antillarum Nelsen 4037 F 97/0.99 Diorygma defectoisidiatum Caceres & Aptroot 28966a' ISE 9/-| | Diorygma defectoisidiatum Caceres & Aptroot 28966b ISE Diorygma toensbergianum Caceres & Aptroot 42003' ISE Diorygma circumfusum Kalb 33922 Herb. Kalb 99/0.92, Diorvema junghuhnii Vhallus MSSRF Diorygma junghuhnii Mycobiont MSSRF O4/- Diorygma Junghuhnit Lumbsch 205391 F 99/1 Ook Diorygma junghuhni Kalb 33254 Herb. Kalb Diorygma junghuhnii Kalb 33931 Herb. Kalb Diorygma junghuhnii Kalb 33937 Herb. Kalb Diorygma sp. Lumbsch 20513a F Diorygma pruinosum Mangold 28g F 99/- Diorygma pruinosum Kalb 26578 Herb. Kalb Diorygma sp. Lumbsch 190821 F 100/1.00, Diorvema poitaei Licking 28538 F 99/100 Diorygma tibellii Licking 28533 F 100/100 Diorygma sipmanii Liicking 14011 F Diorygma microsporum Licking 26504 F 97/0.99- Diorygma minisporum Lumbsch 19543v F 100/1.00) | Diorygma aff. minisporum Medeiros 2106 DUKE Diorygma minisporum Licking 26564 F 100/1.00-— Glyphis cicatricosa Lumbs Glyphis cicatricosa Liicking 0.02 Figure 1. RAxML analysis based on combined LSU and mtSSU sequence data. Bootstrap support values for maximum likelihood (ML = 75%) and Bayesian posterior probabilities (PP = 0.90) are shown near the nodes as ML/PP. Glyphis cica- tricosa (LUcking 28047 F and Lumbsch 195280 F) is used as the outgroup taxon. Newly generated sequences are shown in bold. Type strains are indicated as '. apex, pale yellowish brown towards base. Epihymenium brown, 10-30 um high. Hymenium hyaline, not inspersed, 150-210 um high, I+ weakly blue-vio- let. Paraphysis anastomosing, filiform, 1-2.5 um wide. Hypothecium weakly yellowish brown, 15-45 um high. Asci fusiform, 118-222 x 37-82 um, I-. Ascospores 1/ascus, hyaline, richly muriform, peripheral and central spore locules of + equal size, ends with gelatinous caps, 24-32 x 5-12 locular, (104—-)125-119(-214) x (30-)36-58(-77) um (x = 152 x 47 um, n = 20), I-. Asexual moprh: not observed. MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 128 Wei Wu et al.: Diorygma from China a yt. . * vw a , ie Ss r «. t ee > ay eer Jah. 4 + rite 2 woh Gor Figure 2. Diorygma guizhouense (KUN-L 0093724, holotype). A-C. Thallus with ascomata; D. Cross section of apothe- cium; E. Cross section of apothecium (in IKI); H, I. Asci; J-L. Ascospores (in water); F, G. Ascospore (in IKI). Scale bars: 1 mm (A-C); 100 um (D); 200 um (E); 50 um (F); 20 um (G, H-L). MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 129 Wei Wu et al.: Diorygma from China Chemistry. Thallus K+ reddish brown, C-, KC+ orange, P+ yellow, TLC: stictic acid, salazinic acid, norstictic acid. Material examined. CHINA, * Guizhou Province, Congjiang County, Yueliang- shan Nature Reserve, 25°20'8.56'N, 108°36'19.23"E, 987 m elev., 24 Oct. 2023, Ze Yang & Bo Liu, Y400 (holotype KUN-L 0093724). Notes. The new species Diorygma guizhouense is characterized by lirelliform apothecia, which are erumpent with a narrow to slightly open disc covered by a white pruina. The exciple is uncarbonized, the hymenium is hyaline, not in- spersed, and reacts I+ blue-violet. The ascospores, one per ascus, are hyaline, richly muriform, with peripheral and central spore locules of approximately equal size, containing 24-32 x 5-12 locular, measuring 125-179 x 36-58 um, I-. Chemically, this species contains stictic, salazinic, and norstictic acids. Phylogenetic analysis based on combined LSU and mtSSU sequence data in- dicates that D. guizhouense is closely related to Diorygma tiantaiense (Fig. 1). A comparison with D. tiantaiense reveals a 0.97% nucleotide difference in the LSU re- gion (7/723 bp). Morphologically, D. guizhouense differs by having a narrowly open disc (vs. fully open) and chemically by having a hymenium that reacts I+ blue-violet (vs. I-). According to the TLC result, the new species contains stictic and salazinic acids rather than only norstictic acid in D. tiantaiense (Cui et al. 2024). According to the taxonomic key provided by Feuerstein et al. (2014), D. guizhouense is morphologically similar to D. dandeliense, which was later syn- onymized to D. karnatakense (Ansil et al. 2023). However, D. guizhouense and D. karnatakense occupy distinct clades in the phylogenetic tree, supporting their separation at the species level. Chemotaxonomically, TLC analysis shows that D. guizhouense contains stictic, salazinic, and norstictic acids, whereas D. karna- takense lacks stictic acid, and the ascospores of D. guizhouense exhibit a negative iodine reaction (I-), in contrast to the I+ violet reaction reported in related species (Ansil et al. 2023). The nucleotide comparison reveals clear differences between the two species: 4.28% (31/725 bp) for LSU and 3.45% (25/725 bp) for mtSSU. Diorygma leigongshanense Wei Wu & S.B. Fu, sp. nov. Index Fungorum: IF903745 Facesoffungi Number: FoF 17549 Fig.*3 Etymology. The specific epithet “/eigongshanense’ refers to the location where the holotype was collected. Holotype. KUN-L 0093725 Description. Sexual morph: Thallus corticolous, crustose, thin, tightly at- tached to the substratum, pale grey to greenish grey, rough, dull, lacking isidia and soredia, prothallus absent. Ascomata lirellate, numerous, oblong to long, + flexuous, simple or with a few branches, measuring 0.7-2.5 mm long and 0.4- 1.2 mm wide. Dise surrounded by entire raised thalline margins, widely open, covered with a thin, pale yellowish pruina. Exciple uncarbonized, basally and laterally brownish. Epihymenium brown, 15-41 um high. Hymenium hyaline, not inspersed, 160-350 um high, I-. Paraphysis anastomosing, filiform, 1-2.5 um wide. Hypothecium brown, 20-48 um high. Asci fusiform, 106-202 x 28-58 um, |-. Ascospores 1/ascus, hyaline, richly muriform, peripheral and central spore MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 130 Wei Wu et al.: Diorygma from China Figure 3. Diorygma leigongshanense (KUN-L 0093725, holotype). A-C. Thallus with ascomata; D. Cross section of apoth- ecium; E-G. Ascus; H-K. Ascospores (in water); L. Ascospore (in IKI). Scale bars: 1 cm (A); 1 mm (B, C); 200 um (D); 20 um (E, F); 50 um (G); 20 um (H-L). MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 131 Wei Wu et al.: Diorygma from China locules of + equal size, 20-42 x 5-10 locular, (95-)119-170(-194) x (22—)27- 46(-54) um (x = 144 x 37 um, n = 20), |-. Asexual moprh: not observed. Chemistry. Thallus K+ reddish brown, C-, KC+ yellow, P+ yellow, TLC: stictic acid, salazinic acid, norstictic acid. Material examined. CHINA, * Guizhou Province, Leishan County, Leigong Mountain National Nature Reserve, 26°22'43.16'N, 108°11'42.65'E, 1681 melev., on bark, 27 Oct. 2023, Ze Yang & Bo Liu, LGS207 (holotype KUN-L 0093725). Notes. This species is characterized by its erumpent lirelliform apothecia, with discs surrounded by entire, raised thalline margins that are widely open and covered with a thin, pale yellowish pruina. The exciple is uncarbonized, and the hymenium is hyaline, non-inspersed, and I-. Spores are single per ascus, hyaline, richly muriform, with peripheral and central spore locules of approx- imately equal size, 20-42 x 5-10 locular, measuring 119-170 x 27-46 um. Chemically, this species contains stictic, salazinic, and norstictic acids. Phylogenetic analysis based on combined LSU and mtSSU sequence data places D. leigongshanense as closely related to Diorygma tiantaiense (Fig. 1). However, Diorygma leigongshanense differs chemically by the presence of stic- tic and salazinic acids (vs. only containing norstictic acid). Morphologically, D. leigongshanense has lirelliform apothecia, in contrast to the oval, open, and raised ascocarps of D. tiantaiense (Cui et al. 2024). Diorygma leigongshanense is morphologically similar to D. rufopruinosum (A.W. Archer) Kalb, Staiger & Elix. However, it can be distinguished by its asco- spore septation pattern: in D. leigongshanense, the peripheral cells and central cells are of similar size, whereas in D. rufopruinosum, the peripheral cells are noticeably smaller. Diorygma leigongshanense contains stictic, salazinic, and norstictic acids, while D. rufopruinosum produces protocetraric acid and lacks stictic acid (Kalb et al. 2004). Morphologically, Diorygma leigongshanense also shares similarities with D. chumphonense Sutjaritturakan & K. Kalb, but it can be distinguished by hav- ing longer ascospores (119-170 um vs. 95-110 um), peripheral cells of equal size to the central ones (vs. peripheral cells distinctly smaller), lacking stictic acid (vs. presence), and the I- (vs. I+ blue-violet) (Sutjaritturakan et al. 2014). Diorygma locitonitrus Wei Wu & S.B. Fu, sp. nov. Index Fungorum: IF903746 Facesoffungi Number: FoF 17550 Fig. 4 Etymology. “locitonitius” combines “loci,” signifying locality, with “tonitius,” the Latin word for thunder, to mean “of the locality of thunder,” denoting the loca- tion where the holotype was found. Holotype. KUN-L 0093723 Description. Sexual morph: Thallus corticolous, crustose, thin, tightly at- tached to the substratum, pale grey to greenish grey, rough, dull, lacking isidia and soredia, prothallus absent. Apothecia lirelliform, scattered or aggregated, erumpent, simple or irregularly branched, curved, and either terminally rounded or acute, measuring 0.5-2.5 mm long and 0.2-0.6 mm wide. Dise narrow to open, covered with a white pruina. Exciple uncarbonized, brown at apex, pale MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 132 Wei Wu et al.: Diorygma from China ee yD . 3 Noa ¥ an ry. me pt Si ae ag eh Ss mA a toe Figure 4. Diorygma locitonitrus (KUN-L 0093723, holotype). A, B. Thallus with ascomata; C. Cross section of apothecium; D. Cross section of apothecium (in IKI); E, F. Ascus; I-L. Ascospores (in water); G, H. Ascospore (in IKI). Scale bars: 1 mm (A, B); 100 ym (C, D); 50 ym (E, H, |, J); 20 ym (F, J-L). MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 133 Wei Wu et al.: Diorygma from China yellowish brown towards base. Epihymenium brown, 10-42 um high. Hymeni- um hyaline, not inspersed, 180-350 um high, I+ weakly blue-violet. Paraphysis anastomosing, filiform, 1-2.5 um wide. Asci fusiform, 112-260 x 30-81 um, I-. Ascospores 1/ascus, hyaline, richly muriform, peripheral cells distinctly smaller than central ones, 26-40/6-15 locular, (105-)117-189(—247) x (25-)33-60(- 76) um (x = 153 x 47 um, n = 20), I-. Asexual moprh: not observed. Chemistry. Thallus K+ reddish brown, C-, KC+ orange, P+ yellow, TLC: const- ictic acid, salazinic acid, norstictic acid. Material examined. CHINA, * Guizhou Province, Leishan County, Leigong Mountain National Nature Reserve, 26°22'53.94"N, 108°11'46.76'E, 1771 m elev., on bark, 27 Oct. 2023, Ze Yang & Bo Liu, LGS256-1 (holotype KUN-L 0093723). Notes. This species is characterized by its lirelliform apothecia, which are erumpent with narrow to open discs covered by a white pruina; the exciple is uncarbonized, and the hymenium is hyaline, not inspersed, and reacts I+ weakly blue-violet. The ascospores, one per ascus, are hyaline, richly muriform, with peripheral cells distinctly smaller than central ones, containing 26-40 x 6-15 locular, measuring 117-189 x 33-60 um, I-. Chemically, this species contains constictic, salazinic, and norstictic acids. Phylogenetic analysis based on combined LSU and mtSSU sequence data places Diorygma locitonitrus as closely related to D. tiantaiense (Fig. 1). Howev- er, nucleotide comparison of the LSU reveals a difference between D. /ocitoni- trus of 0.80% (6/758 bp) between the two species. The new taxon is chemically distinct from the presence of both norstictic and salazinic acids, while D. tian- taiense contains only norstictic acid. In addition, the hymenium of D. locitoni- trus exhibits a weakly I+ blue-violet reaction in Lugol's solution, in contrast to the I- reaction in D. tiantaiense (Cui et al. 2024). Diorygma locitonitrus is morphologically similar to D. chumphonense but dif- fers in the larger ascospores (117-189 x 33-60 um vs. 95-110 x 37-40 pm) and the iodine reaction of the hymenium (I-, vs. + weakly violet). Additionally, the ascospores of D. /ocitonitrus are |-, in contrast to the I+ violet reaction ob- served in D. chumphonense (Sutjaritturakan et al. 2014). Diorygma weii Wei Wu & S.B. Fu, sp. nov. Index Fungorum: IF903747 Facesoffungi Number: FoF17617 FIG. Etymology. The species epithet “weii” honors Professor Jiangchun Wei (Chi- nese Academy of Sciences), a venerable lichenologist, for his pioneering con- tributions to lichenology in China. Holotype. KUN-L 0093727 Description. Sexual morph: Thallus corticolous, crustose, thin, tightly at- tached to the substratum, milky white, with a slight greenish tint, rough, dull, lacking isidia and soredia, prothallus absent. Apothecia lirelliform, prominent, stellately branched, curved, and either terminally rounded or acute, measuring 2-7 mm long and 0.1-0.4 mm wide. Dise closed to slit-like, covered with a thin white pruina. Proper margin conspicuous. Exciple uncarbonized, brown at apex, pale yellowish brown towards base. Hymenium hyaline, not inspersed, MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 134 Wei Wu et al.: Diorygma from China a pn So -/ cos ail Figure 5. Diorygma weii (KUN-L 0093727, holotype). A-C. Thallus with ascomata; D. Cross section of apothecium; E. Cross section of apothecium (in IKI); F-H. Ascus; I, J. Ascospores (in water); K. Ascospore (in IKI). Scale bars: 1 mm (A-C); 50 um (D); 100 um (E); 20 um (F-K). MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 135 Wei Wu et al.: Diorygma from China 150-210 um high, I+ violet. Paraphysis anastomosing, filiform, 1-2 um wide. Asci fusiform, 60-155 x 18-45 um, I- or I+ violet. Ascospores 1/ascus, hya- line, richly muriform, peripheral and central spore locules of + equal size, ends with gelatinous caps, 24—28/6-9 locular, (55—)73-119(-142) x (11-)18-35(- 41) um (x = 96 x 27 um, n = 20), I+ violet. Asexual moprh: not observed. Chemistry. Thallus K+ reddish brown, C-, KC+ yellow, P+ yellow, TLC: Norst- ictic acid. Material examined. CHINA, * Guizhou Province, Leishan County, Leigong Moun- tain National Nature Reserve, 26°20'33.94'N, 108°17'23.94"E, 831 m elev., on the bark, 25 Oct. 2023, Ze Yang & Bo Liu, Coll. No. LGS57 (holotype KUN-L 0093727). Notes. This new species is characterized by its lirelliform apothecia, which are prominent with a closed-to-slit-like disc covered by a thin white pruina. The exciple is uncarbonized, and the hymenium is hyaline, not inspersed, and reacts I+ violet. Spores are single per ascus, hyaline, richly muriform, with peripheral and central spore locules of approximately equal size, 24-28 x 6-9 locular, measuring 73-119 x 18-35 um, I+ violet. Chemically, this species contains only norstictic acid. Phylogenetic analysis based on the combined data of LSU and mtSSU sequenc- es indicates that D. weii forms a clade with D. karnatakense (Fig. 1). However, nucleotide divergence in mtSSU between the two species is 3.52% (23/653 bp). Morphologically, D. weii has asci containing a single spore, versus 1—8-spored asci in D. karnatakense. Chemically, D. weii only produces norstictic acid, whereas D. karnatakense contains both norstictic and salazinic acids (Ansil et al. 2023). Diorygma weii shares morphological similarities with Diorygma inaequale and D. dealbatum B.O. Sharma & Makhija. However, both D. inaequale and D. dealbatum contain both salazinic and norstictic acids, whereas the new spe- cies produces only norstictic acid (Sharma and Makhija 2009a). Discussion Morphological features and chemical compounds are generally used in the classification of Diorygma species (Kalb et al. 2004). Chemically, most species exhibit remarkable diversity in secondary metabolites—such as norstictic acid, stictic acid, cryptostictic acid, and protocetraric acid—which serve as key tax- onomic markers. Typically, a single species produces one or several of these characteristic metabolites (Kalb et al. 2004). With the rapid advancement of molecular technologies, phylogenetic analyses have become an indispensable tool in species identification and evolutionary studies. Molecular data provide objective genetic evidence that enables the precise differentiation of morpho- logically similar or cryptic species, help clarify taxonomic uncertainties includ- ing synonymies, and facilitate the discovery of new taxa. Phenotypic charac- teristics alone are sometimes insufficient for resolving taxonomic ambiguities due to environmental influences and developmental stage. In contrast, genetic data are relatively stable. DNA sequences from LSU and mtSSU are usually considered reliable molecular markers, and phylogenetic trees based on these genes can confirm the monophyletic nature of species defined by morphology and correct misclassifications caused by overlapping features of sporangia or ascospores within Diorygma (Cui et al. 2024; Aptroot et al. 2023). Therefore, this study adopts an integrative taxonomic approach, combining phylogenet- MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 136 Wei Wu et al.: Diorygma from China ic analyses, morphological characteristics, and chemical profiling to achieve a comprehensive and accurate classification of new Diorygma species. Most Diorygma species have stable phylogenetic positions with stronger sta- tistical support in multigene datasets than in single-gene analyses (Ansil et al. 2023). The phylogenetic tree based on LSU + mtSSU sequence data provided high-resolution species delimitation within Diorygma. Diorygma guizhouense, D. leigongshanense, and D. locitonitrus formed a highly supported clade (ML/ PP = 90/0.90) with D. tiantaiense. Although D. weii and D. karnatakense formed a distinct clade, this relationship received weak support (ML/PP < 70/0.90). All four proposed species occupy distinct phylogenetic positions. Approximately 90 Diorygma species are currently recognized and categorized by ascospore number into two morphological groups (Table 2). Among the 38 monosporate species, our four new taxa all belong to this group. Thirteen mono- sporate species produce salazinic acid—a feature shared by D. guizhouense and D. leigongshanense. These two species are further distinguished by co-occurring salazinic and stictic acids, a combination previously documented only in D. an- gusticarpum Sutjaritturakan & Kalb and D. salazinicum Sutjaritturakan & Kalb. Morphological and chemical comparisons reveal that D. guizhouense can be distinguished from D. angusticarpum by its larger ascospores (125-179 x 36-58 um vs. 90-110 x 30-37 um) and the presence of norstictic acid (vs. cryptostictic acid) (Sutjaritturakan et al. 2014). Additionally, D. guizhouense dif- fers from D. salazinicum in having a narrower disc (vs. widely opened discs), a weakly I+ blue-violet hymenium reaction (vs. I-), and the presence of norstictic acid (vs. cryptostictic acid) (Sutjaritturakan et al. 2014). Comparative analysis reveals that D. leigongshanense exhibits unique diag- nostic features when contrasted with similar species. Compared to D. angus- ticarpum, the new species displays markedly larger ascospores (119-170 x 27-46 um vs. 90-110 x 30-37 um), a distinctly broader disc (vs. slit-like), an I- hymenium (vs. I+ violet), and contains norstictic acid (vs. cryptostictic acid) (Sutjaritturakan et al. 2014). These chemical and morphological differences also distinguish D. leigongshanense from D. salazinicum, particularly in the io- dine reaction patterns of the ascospores (I- vs. I+ violet) and secondary metab- olite profiles (norstictic acid vs. cryptostictic acid) (Sutjaritturakan et al. 2014). Taxonomic evaluation of ascospore septation (cell size) in monosporate Diorygma species revealed two distinct morphological types: those with pe- ripheral cells smaller than central cells and those with cells of equal size. The newly described D. locitonitrus possesses the former pattern, a characteristic shared with nine other monosporate species. Notably, only three previously documented species—D. reniforme (Fée) Kalb, Staiger & Elix, D. rufopruinosum, and D. salvadoriense Kalb, Staiger & Elix—exhibit both this septation pattern and the presence of salazinic acid. Diorygma locitonitrus can be distinguished from these chemically similar species by the absence of protocetraric acid (vs. present in D. reniforme and D. rufopruinosum) and the absence of pycnidia (vs. present in D. salvadoriense) (Kalb et al. 2004). These consistent differences in secondary metabolite profiles and reproductive structures provide robust crite- ria for delimiting D. locitonitrus from related taxa. Among monosporate Diorygma species, seven taxa—D. australasicum (Elix) Licking, Elix & A.W. Archer, D. isabellinum (Zahlbr.) Z.F. Jia & Liicking, D. longil- irellatum B.O. Sharma & Makhija, D. roseopruinatum Papong, Lucking & Parn- MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 137 Wei Wu et al.: Diorygma from China men, D. soozanum (Zahlbr.) M. Nakan. & Kashiw., D. spilotum (Stirt.) Pushpi Singh & Kr.P. Singh, and D. tiantaiense—exclusively produce norstictic acid (Ta- ble 2), warranting detailed comparison with D. weii, which shares this chemical profile. Diorygma weii is distinguished by its unique thallus morphology (milky white with a slight greenish tint) and apothecial features (stellately branched with closed to slit-like discs). Key diagnostic characters that distinguish D. weii from related species include: (1) From D. roseopruinatum by thallus color (milky white vs. light grey), and disc exposure and pruinosity (closed, slit-like white pruinose vs. narrow brown with pink-red pruina) (Papong et al. 2014); (2) From D. australasicum by the absence of isidia (Archer and Elix 2009); (3) From D. spilotum by branching of lirellae (prominent stellately branched vs. simple im- mersed) and disc pruinosity (white pruinose vs. epruinose) (Singh and Singh 2017); (4) From D. isabellinum by branching of lirellae (stellately vs. single/rare- ly branched) and iodine reaction (I+ violet hymenium vs. I-) (Jia and Liicking 2017); (5) From D. longilirellatum by thallus color (milky white vs. greenish-grey) and disc exposure (closed vs. slightly open) (Sharma and Makhija 2009b); (6) From D. tiantaiense by smaller ascospores (73-119 x 18-35 um vs. 120-210 x 35-60 um), disc exposure (closed vs. open), and iodine reactions (I+ violet vs. I- for both hymenium and ascospores) (Cui et al. 2024); (7) From D. soozanum by narrower ascospores (73-119 x 18-35 um vs. 110-140 x 35-45 um) and disc exposure (closed vs. initially narrow then wide) (Kalb et al. 2004). This study describes four new species of the lichen genus Diorygma from Chi- na. Each species exhibits distinctive morphological features and secondary me- tabolite profiles, confirmed through colorimetric tests and thin-layer chromatog- raphy. These findings contribute to a deeper understanding of Diorygma diversity in China and underscore the value of combining traditional taxonomy with mo- lecular and chemotaxonomic approaches. A comprehensive species checklist is also provided to aid in the identification and comparison of known Diorygma taxa. Additional information Conflict of interest The authors have declared that no competing interests exist. Ethical statement No ethical statement was reported. Use of Al No use of Al was reported. Funding No funding was reported. Author contributions Wei Wu and Shao-Bin Fu designed the experiments and structured the manuscript. Wei Wu conducted the experiments, analyzed the data, and drafted the manuscript. He-Yun Bo and Lin-Shan Chai conducted part of the molecular and chemical experiments. Qing- Feng Meng, Shu-Hao Jiang, Ruvishika S. Jayawardena, and Shao-Bin Fu contributed to data analysis and manuscript revision. MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 138 Wei Wu et al.: Diorygma from China Author ORCIDs Wei Wu © https://orcid.org/0009-0009-1 191-6667 Shu-Hao Jiang ® hitps://orcid.org/0009-0009-8619-2667 Lin-Shan Chai © https://orcid.org/0009-0005-2458-0454 He-Yun Bo © https://orcid.org/0009-0003-8641-1619 Ruvishika S. Jayawardena © https://orcid.org/0000-0001-7702-4885 Shao-Bin Fu © https://orcid.org/0000-0001-9932-1346 Qing-Feng Meng ® https://orcid.org/0000-0001-9814-8238 Data availability All of the data that support the findings of this study are available in the main text. References Ansil PA, Rajeshkumar KC, Sharma B, Liicking R, Hawksworth DL (2023) Phylogenetic placement and reappraisal of Diorygma karnatakense including the new synonym, Diorygma dandeliense, from Maharashtra, India. Lichenologist 55(2): 59-67. https:// doi.org/10.1017/S0024282923000087 Aptroot A, Feuerstein S (2020) New Graphidaceae from South and Central Brazil. Ar- chive for Lichenology 16: 1-11. Aptroot A, Thor G, Liicking R, Elix JA, Chaves JL (2009) The lichen genus Herpothallon reinstated. Bibliotheca Lichenologica 99: 19-66. Aptroot A, Liicking R, Caceres MES (2023) New species and records of Graphidace- ae and Gomphillaceae (lichenized fungi) from Brazil. Plant and Fungal Systematics 68(2): 249-261. https://doi.org/10.35535/pfsyst-2023-0010 Aptroot A, Licking R, Caceres MES (2024) New species, records and combinations of Graphidaceae (Lichenized Fungi) from Brazil. The Bryologist 127(1): 22-55. https:// doi.org/10.1639/0007-2745-127.1.022 Archer AW (2003) New species in the lichen family Graphidaceae (Ascomycota) from Australia and the Solomon Islands. Mycotaxon 88: 143-148. Archer AW (2005) Australian species in the genus Diorygma (Graphidaceae). Austral- asian Lichenology 56: 10-11. https://doi.org/10.7751/telopea20055705 Archer AW (2007) Key and checklist for the lichen family Graphidaceae (lichenised As- comycota) in the Solomon Islands. Systematics and Biodiversity 5(1): 9-22. https:// doi.org/10.1017/S1477200006002040 Archer AW, Elix JA (2008) Three new species in the Australian Graphidaceae (lichenized Ascomycota). Australasian Lichenology 63: 26-29. Archer AW, Elix JA (2009) A new species, new combination, and new report in the Aus- tralian Graphidaceae. Australasian Lichenology 65: 24-29. Archer AW, Elix JA (2017) A new species of Diorygma (Graphidaceae, lichenized Ascomyco- ta), and notes on Diaphorographis queenslandica. Australasian Lichenology 81: 93-98. Caceres MES (2007) Corticolous crustose and microfoliose lichens of northeastern Bra- zil. Libri Botanici 22: 1-168. Capella-Gutiérrez S, Silla-Martinez JM, Gabaldon T (2009) TrimAl: A tool for automat- ed alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25(15): 1972-1973. https://doi.org/10.1093/bioinformatics/btp348 Cui C, Li YJ, Xu JH, Zhao X, Jia ZF (2024) Diorygma tiantaiense sp. nov. and a check- list and key to Diorygma species from China. Diversity 16(4): e213. https://doi. org/10.3390/d16040213 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 139 Wei Wu et al.: Diorygma from China Culberson CF (1972) Improved conditions and new data for identification of lichen prod- ucts by standardized thin-layer chromatographic method. Journal of Chromatogra- phy A 72(1): 113-125. https://doi.org/10.1016/0021-9673(72)80013-X Lima EL, Maia LC, Aptroot A, Caceres MES (2013) New lichen species from Vale do Catimbau, Pernambuco, Brazil. The Bryologist 116(4): 327-329. https://doi. org/10.1639/0007-2745-116.4.327 Lima EL, Maia LC, Martins MCB, Da Silva NL, Liicking R, Caceres MES (2019) Five new species of Graphidaceae from the Brazilian Northeast, with notes on Diorygma alago- ense. The Bryologist 122(3): 414-422. https://doi.org/10.1639/0007-2745-122.3.414 Eschweiler FG (1824) Systema Lichenum, Genera Exhibens rite distincta. Pluribus Novis Adaucta, 26 pp. Eschweiler FMD (1833) Ordo secundus Lichens descripsit (Vol. 1). Flora Brasiliensis seu Enumeratio Plantarum in Brasilia, 51-293. Feuerstein SC, Cunha-Dias IPR, Aptroot A, Eliasaro S, Caceres MES (2014) Three new Diorygma (Graphidaceae) species from Brazil, with a revised world key. Lichenologist 46(6): 753-761. https://doi.org/10.1017/S002428291400036X Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New al- gorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology 59(3): 307-321. https://doi. org/10.1093/sysbio/syq010 Hoang DT, Chernomor O, Von Haeseler A, Minh BQ, Vinh LS (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35(2): 518- 522. https://doi.org/10.1093/molbev/msx281 Jayasiri S, Hyde K, Ariyawansa H, Bhat J, Buyck B, Cai L, Dai Y, Abd-Elsalam K, Ertz D, Hidayat | (2015) The faces of fungi database: Fungal names linked with mor- phology, molecular and human attributes. Fungal Diversity 74(1): 3-18. https://doi. org/10.1007/s13225-015-0351-8 Jia ZF, Licking R (2017) Resolving the species of the lichen genus Graphina Mull. Arg. in China, with some new combinations. MycoKeys 25: 13-29. https://doi.org/10.3897/ mycokeys.25.13154 Joshi S, Jayalal U, Oh SO, Koh YJ, Nguyen TT, Dzung NA, Hur JS (2013) New species and new records in the family Graphidaceae (Ascomycota: Ostropales) from Vietnam. Lichenologist 45(5): 599-609. https://doi.org/10.1017/S002428291300025X Kalb K (2020) New or otherwise interesting lichens. VII, including a world key to the lichen genus Heiomasia. Archive for Lichenology 15: 1-18. Kalb K, Staiger B, Elix JA (2004) A monograph of the lichen genus Diorygma — a first attempt. Symbolae Botanicae Upsalienses 34(1): 133-181. Kalyaanamoorthy S, Minh BQ, Wong TKF, Von Haeseler A, Jermiin LS (2017) ModelFind- er: Fast model selection for accurate phylogenetic estimates. Nature Methods 14(6): 587-589. https://doi.org/10.1038/nmeth.4285 Katoh K, Standley D (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution 30(4): 772-780. https://doi.org/10.1093/molbev/mst010 Kraichak E, Lucking R, Aptroot A, Beck A, Dornes P, John V, Lendemer JC, Nelsen MP Neuwirth G, Nutakki A (2015) Hidden diversity in the morphologically variable script lichen (Graphis scripta) complex (Ascomycota, Ostropales, Graphidaceae). Organ- isms, Diversity & Evolution 15: 447-458. https://doi.org/10.1007/s13127-015-0219-5 Li J, Jia ZF (2016) Diorygma fuscum sp. nov. from China. Mycotaxon 131(3): 717-721. https://doi.org/10.5248/131.717 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 140 Wei Wu et al.: Diorygma from China Lumbsch HT, Ahti T, Altermann S, De Paz GA, Aptroot A, Arup U, Pena AB, Bawingan PA, Benatti MN, Betancourt L, Bjork CR, Boonpragob K, Brand M, Bungartz F, Caceres MES, Candan M, Chaves JL, Clerc P Common R, Coppins BJ, Crespo A, Dal-Forno M, Divakar PK, Duya MV, Elix JA, Elvebakk A, Fankhauser JD, Farkas E, Ferraro LI, Fischer E, Galloway DJ, Gaya E, Giralt M, Goward T, Grube M, Hafellner J, Hernandez JE, Campos MDH, Kalb K, Karnefelt |, Kantvilas G, Killmann D, Kirika P Knudsen K, Komposch H, Kondratyuk S, Lawrey JD, Mangold A, Marcelli MP Mccune B, Messuti MI, Michlig A, Gonzalez RM, Moncada B, Naikatini A, Nelsen MP, Ovstedal DO, Palice Z, Papong K, Parnmen S, Perez-Ortega S, Printzen C, Rico VJ, Plata ER, Robayo J, Rosabal D, Ruprecht U, Allen NS, Sancho L, De Jesus LS, Vieira TS, Schultz M, Sea- ward MRD, Serusiaux E, Schmitt |, Sipman HJM, Sohrabi M, Sochting U, Sogaard MZ, Sparrius LB, Spielmann A, Spribille T, Sutjaritturakan J, Thammathaworn A, Thell A, Thor G, Thus H, Timdal E, Truong C, Turk R, Tenorio LU, Upreti DK, Van Den Boom P, Rebuelta MV, Wedin M, Will-Wolf S, Wirth V, Wirtz N, Yahr R, Yeshitela K, Ziemmeck F, Wheeler T, Liicking R (2011) One hundred new species of lichenized fungi: A signa- ture of undiscovered global diversity. Phytotaxa 18: 1-127. https://doi.org/10.11646/ phytotaxa.18.1.1 Makhija U, Chitale G, Sharma B (2009) New species and new records of Diorygma (Graphidaceae) from India: Species with convergent exciples. Mycotaxon 109(1): 379-392. https://doi.org/10.5248/109.379 Mangold A, Martin MP, Licking R, Lumbsch HT (2008) Molecular phylogeny suggests synonymy of Thelotremataceae within Graphidaceae (Ascomycota: Ostropales). Tax- on 57(2): 476-486. https://doi.org/10.2307/25066016 Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for infer- ence of large phylogenetic trees. 2010 gateway computing environments workshop (GCE). IEEE, 8 pp. https://doi.org/10.1109/GCE.2010.5676129 Mohabe S, Nayaka S, Reddy AM, Devi BA (2015) Diorygma kurnoolensis (Graphidaceae), a new saxicolous lichen species from Andhra Pradesh, India. Geophytology 45: 47-50. Miller J (1895) Thelotremeae et Graphideae novae quas praesertim ex hb. Reg. Kewen- si exponit. Botanical Journal of the Linnean Society 30(211): 451-463. https://doi. org/10.1111/j.1095-8339.1895.tb02420.x Nelsen MP Licking R, Andrew CJ, Plata ER, Chaves JL, Caceres MES, Ventura N (2012) Dismantling Herpothallon Herpothallon antillarum (Arthoniomycetes: Arthoniaceae) is a member of the genus Diorygma (Lecanoromycetes: Graphidaceae). The Bryolo- gist 115: e313. https://doi.org/10.1639/0007-2745-115.2.313 Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Bi- ology and Evolution 32(1): 268-274. https://doi.org/10.1093/molbev/msu300 Papong KB, Liicking R, Kraichak E, Parnmen S, Von Konrat M, Lumbsch HT (2014) Twen- ty-three new species in the lichen family Graphidaceae from New Caledonia (Ost- ropales, Ascomycota). Phytotaxa 189(1): 204-231. https://doi.org/10.11646/phyto- taxa.189.1.15 Rivas Plata E, Licking R (2013) High diversity of Graphidaceae (lichenized ascomycota: Ostropales) in Amazonian Peru. Fungal Diversity 58: 13-32. https://doi.org/10.1007/ $13225-012-0172-y Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539-542. https://doi.org/10.1093/sysbio/sys029 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 141 Wei Wu et al.: Diorygma from China Schumm F, Aptroot A (2024) Rond6nia. Brazilian Lichens 7: 1-668. Seavey F, Seavey J (2014) Four new species and sixteen new lichen records for North America from Everglades National Park. The Bryologist 117(4): 395-404. https://doi. org/10.1639/0007-2745-117.4.395 Sharma B, Khadilkar P (2012) Four new species of Diorygma from India. Mycotaxon 119: 1-10. https://doi.org/10.5248/119.1 Sharma B, Makhija U (2009a) Four new species in the lichen genus Diorygma. Mycotax- on 107(1): 87-94. https://doi.org/10.5248/107.87 Sharma B, Makhija U (2009b) New species and new reports of Diorygma lichenized Ascomycotina, Graphidaceae from India. Mycotaxon 109: 209-217. https://doi. org/10.5248/109.209 Singh P, Singh KP (2017) New combinations in the family Graphidaceae (lichenized Ascomycota: Ostropales) from India. Lichenologist 49(5): 527-533. https://doi. org/10.1017/S002428291 7000330 Singh P Singh KP (2020) New combinations and synonyms in Graphidaceae (lichenized Ascomycota) from India. Lichenologist 52(3): 251-256. https://doi.org/10.1017/ $0024282920000043 Sipman HJM (2014) New species of Graphidaceae from the Neotropics and Southeast Asia. Phytotaxa 189(1): 289-311. https://doi.org/10.11646/phytotaxa.189.1.21 Staiger B (2002) Die Flechtenfamilie Graphidaceae. Bibliotheca Lichenologica 85: 1-526. Stamatakis A (2014) RAXxML version 8: A tool for phylogenetic analysis and post-analy- sis of large phylogenies. Bioinformatics 30(9): 1312-1313. https://doi.org/10.1093/ bioinformatics/btu033 Sutjaritturakan J, Saipunkaew W, Boonpragob K, Kalb K (2014) New species of Graphi- daceae (Ostropales, Lecanoromycetes) from Southern Thailand. Phytotaxa 189(1): 312-324. https://doi.org/10.11646/phytotaxa.189.1.22 Swarnalatha G (2021) A new species of Diorygma (Graphidaceae) from India. Botanical Survey of India Archive for Lichenology 26: 1-4. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172(8): 4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990 Zeng XY, Tan TJ, Tian FH, Wang Y, Wen TC (2023) OFPT: A one-stop software for fungal phylogeny. Mycosphere: Journal of Fungal Biology 14(1): 1730-1741. https://doi. org/10.5943/mycosphere/14/1/20 Zhurbenko MP, Diederich P (2024) One new genus and six new species of lichenicolous hyphomycetes. Herzogia 37(1): 48-61. https://doi.org/10.13158/heia.37.1.2024.48 Zoller S, Scheidegger C, Sperisen C (1999) PCR primers for the amplification of mito- chondrial small subunit ribosomal DNA of lichen-forming Ascomycetes. Lichenolo- gist (London, England) 31(5): 511-516. https://doi.org/10.1006/lich.1999.0220 MycoKeys 121: 111-142 (2025), DOI: 10.3897/mycokeys.121.157714 142